Enhancement of downy mildew disease resistance in pearl millet by the Gapp7 bioactive compound produced by Ganoderma applanatum

Jogaiah, S. and Shetty, H. S. and Ito, Shin-ichi and Tran, Lam-Son Phan (2016) Enhancement of downy mildew disease resistance in pearl millet by the Gapp7 bioactive compound produced by Ganoderma applanatum. Plant Physiology and Biochemistry, 105. 109 - 117.

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Pearl millet (Pennisetum glaucum) stands sixth among the most important cereal crops grown in the semi-arid and arid regions of the world. The downy mildew disease caused by Sclerospora graminicola, an oomycete pathogen, has been recognized as a major biotic constraint in pearl millet production. On the other hand, basidiomycetes are known to produce a large number of antimicrobial metabolites, providing a good source of anti-oomycete agrochemicals. Here, we report the discovery and efficacy of a compound, named Gapp7, purified from Ganoderma applanatum on inhibition of growth and development of S. graminicola, as well as the effects of seed treatment with Gapp7 on protection of pearl millet from downy mildew. Gapp7 consistently demonstrated remarkable effects against S. graminicola by recording significant inhibition of sporangium formation (41.4), zoospore release (77.5) and zoospore motility (91). Analyses of Gapp7 compound using two-dimensional nuclear magnetic resonance spectroscopy and liquid chromatography-mass spectrometry revealed its close resemblance to metominostrobin, a derivative of strobilurin group of fungicides. Furthermore, the Gapp7 was shown to stably maintain the inhibitory effects at different temperatures between 25 and 80 °C. In addition, the anti-oomycete activity of Gapp7 was fairly stable for a period of at least 12 months at 4 °C and was only completely lost after being autoclaved. Seed treatment with Gapp7 resulted in a significant increase in disease protection (63) under greenhouse conditions compared with water control. The identification and isolation of this novel and functional anti-oomycete compound from G. applanatum provide a considerable agrochemical importance for plant protection against downy mildew in an environmentally safe and economical manner.

Item Type: Article
Uncontrolled Keywords: Anti-oomycete compound, Downy mildew disease resistance, , LC-MS, Pearl millet, , 2D NMR
Subjects: B Life Science > Biotechnology
Divisions: Department of > Biotechnology
Depositing User: Manjula P Library Assistant
Date Deposited: 20 Jun 2019 05:58
Last Modified: 20 Jun 2019 05:58
URI: http://eprints.uni-mysore.ac.in/id/eprint/3464

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