Ion-pair extractive spectrophotometric assay of terbinafine hydrochloride in pharmaceuticals and spiked urineusing bromocresol purplen.

Salem Qarah, A. and Basavaiah, K. and Swamy, N. (2016) Ion-pair extractive spectrophotometric assay of terbinafine hydrochloride in pharmaceuticals and spiked urineusing bromocresol purplen. Journal of Applied Spectroscopy, 83 (4). pp. 694-702. ISSN 1573-8647

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Abstract

Two simple, rapid, selective, and sensitive methods were developed and validated for the determination of terbinafi ne hydrochloride (TBH) in pharmaceuticals and urine. The fi rst method (method A) is based on the formation of a yellow ion-pair complex of TBH and bromocresol purple (BCP), a sulfonephthalein dye, in Walpole buffer of pH 3.61, which was extracted into chloroform and investigated at 420 nm. For the second method (method B) the drug–dye ion-pair was broken in alkaline KOH medium, and the resulting free dye color was measured at 610 nm. All variables were studied to optimize the reaction conditions. The regression analysis of Beer’s law plots showed good correlation in the concentration ranges of 1–10 and 0.1–2.0 μg/mLfor method A and method B, respectively. Molar absorptivity values were 2.99 × 104, and 1.51×105 L/(mol × cm) for measurements by these methods. The methods were also validated for limits of detection (LOD) and quantifi cation (LOQ), intra-day and inter-day accuracy and precision, selectivity, robustness and ruggedness. The composition of the ion-pair (drug–dye) used in the method A was found to be 1:1 by both mole-ratio and Job's methods. The developed methods were applied to tablets, and the results were in good agreement with the label claim and those of the reference method. Because of its high sensitivity, method A was applied to spiked human urine with percent recoveries in the range 96.58-107.3 and a standard deviation <2%

Item Type:	Article
Subjects:	C Chemical Science > Chemistry
Divisions:	Department of > Chemistry
Depositing User:	Manjula P Library Assistant
Date Deposited:	19 Jun 2019 08:18
Last Modified:	19 Jun 2019 08:18
URI:	http://eprints.uni-mysore.ac.in/id/eprint/3405

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