Comparative study on plant latex proteases and their involvement in hemostasis: A special emphasis on clot inducing and dissolving properties

Rajesh, R. and Shivaprasad, H. V. and Raghavendra Gowda, C. D. and Nataraju, A. and Dhananjaya, B. L. and Vishwanath, B. S. (2007) Comparative study on plant latex proteases and their involvement in hemostasis: A special emphasis on clot inducing and dissolving properties. Planta Medica, 73 (10). pp. 1061-1067.

Full text not available from this repository. (Request a copy)
Official URL: http://dx.doi.org/10.1055/s-2007-981575

Abstract

In the present study we compared the clot inducing and dissolving properties of Calotropis gigantea R. Br. (Asclepiadaceae), Synadenium grantii Hook. f. (Euphorbiaceae) and Wrightia tinctoria R. Br. (Apocynaceae) latex extracts. All the three latex extracts hydrolyzed casein, fibrinogen and crude fibrin dose-dependently. The proteolytic action on fibrinogen subunity was in the order of A alpha > B beta > gamma All extracts exhibited procoagulant activity as assayed by re-calcification time. However, thrombin like activity is restricted to C. gigantea. In addition, the extracts dose-dependently hydrolyzed blood and plasma clots. Furthermore, the hydrolyzing pattern of fibrin in the plasma clot was substantiated by SIDS-PAGE. The extracts hydrolyzed all the subunits (a polymer, a-chains, gamma-gamma dimer and beta-chain) of fibrin efficiently. Both fibrinogenolytic and fibrinolytic activity potency of the extracts were in the order of C. gigantea > S. grantii > W. tinctoria. Among the three latices, C. gigantea is toxic with a minimum hemorrhagic dose (MHD) of > 75 mu g, whereas S. grantii and W. tinctorla latex extracts were non-toxic and did not induce any hemorrhagic effect at the tested dose (> 200 mu g). The proteolytic activity of C. gigantea latex extract on different substrates was inhibited by IAA. On the other hand, the proteolytic activities of S. grantii and W. tinctorla were inhibited by PMSF. Thus, this study provides the basis for the probable action of plant latex proteases to stop bleeding and effect wound healing as exploited in folk medicine.

Item Type: Article
Uncontrolled Keywords: Calotropis gigantea; Asclepiadaceae; Synadenium grantii; Euphorbiaceae; Wrightia tinctoria; Apocynaceae hemostasis; wound healing; folk medicine
Subjects: Agriculture Biological Sciences > Biochemistry
University of Mysore > PG Campuses > Manasagangotri, Mysore > Agriculture Biological Sciences > Biochemistry
Divisions: Constituent Colleges > Yuvaraja's College Mysore > Biochemistry
Depositing User: Users 7 not found.
Date Deposited: 04 Apr 2013 11:44
Last Modified: 07 Nov 2013 05:43
URI: http://eprints.uni-mysore.ac.in/id/eprint/3402

Actions (login required)

View Item View Item