Sensitive spectrophotometric determination of metoclopramide hydrochloride in dosage forms and spiked human urine using vanillin

Devi, O. Zenita and Basavaiah, K. and Vinay, K. B. and Revanasiddappa, H. D. (2016) Sensitive spectrophotometric determination of metoclopramide hydrochloride in dosage forms and spiked human urine using vanillin. Arabian Journal of Chemistry, 9. S64 - S72.

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Abstract

A new spectrophotometric method which is simple, sensitive, selective and rapid is described for the determination of metoclopramide hydrochloride (MCP) in bulk drug and in dosage forms using vanillin as the chromogenic agent. The method is based on the condensation reaction between primary aromatic amine group present in MCP with aromatic aldehyde, vanillin to produce an intense yellow colored product. The resulting Schiff’s base shows an absorption maximum at 410nm and the reaction product is stable for more than one day. The reaction was carried out in acetic acid and perchloric acid medium. Beer’s law was obeyed in the concentration range 1.5–15.0μgml−1 MCP with a molar absorptivity of 1.89×104lmol−1cm−1. The limit of detection (LOD) and limit of quantification (LOQ) were found to be 0.51 and 1.55μgml−1, respectively. The method was statistically evaluated by calculating percent relative error ( RE) for accuracy and percent relative standard deviation ( RSD) for precision, and was applied successfully to the determination of MCP in tablets, in injection and also in spiked human urine. No interference was observed from common additives found in pharmaceutical preparations. The results obtained by the proposed method were validated statistically by comparing the results with those of the reference method by applying the Student’s t-test and F-test. The accuracy and reliability of the method were further ascertained by performing recovery tests via standard-addition technique.

Item Type:	Article
Uncontrolled Keywords:	Metoclopramide, Vanillin, Schiff’s base, Tablets, Injection, Spiked human urine
Subjects:	C Chemical Science > Chemistry
Divisions:	Department of > Chemistry
Depositing User:	Manjula P Library Assistant
Date Deposited:	19 Jun 2019 05:27
Last Modified:	19 Jun 2019 05:27
URI:	http://eprints.uni-mysore.ac.in/id/eprint/3385

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