New methods for the determination of zidovudine in pharmaceuticals using N-bromosuccinimide, methyl orange and indigo carmine as reagents

Anil Kumar, U. R. and Basavaiah, K. (2007) New methods for the determination of zidovudine in pharmaceuticals using N-bromosuccinimide, methyl orange and indigo carmine as reagents. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES INDIA SECTION A-PHYSICAL SCIENCES, 77A (Part 4). pp. 301-308.

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Titrimetric and spectrophotometric methods are used for the assay of zidovudine (ZDV) in bulk drug and in tablets/capsules using N-bromosuccinimide (NBS) and dyes methyl orange and indigo can-nine as reagents. In titrimetry, ZDV is treated with a known excess NBS in acid medium and after the oxidation reaction is complete, the unreacted oxidant is back-titrated iodometrically. Spectrophotometric methods involve the addition of a known excess of NBS to ZDV in acid medium, followed by determination of unreacted oxidant by reacting with a fixed amount of either methyl orange and measuring the absorbance at 520 nm (method A) or indigo carmine and measuring the absorbance at 610 nm (method B). In both the methods, the amount of NBS reacted corresponds to the amount of ZDV. Titrimetric method is applicable over a range 4-15 mg and the reaction stoichiometry is found to be 1:2 (ZDV : NBS). In spectrophotometry, the measured absorbance is found to increase linearly with the concentration of ZDV. The systems obey Beer's law for 0.5 - 6.0 mu g ml(-1) and 2.5 - 20.0 mu g ml(-1) for method A and method B respectively. The apparent molar absorptivities are calculated to be 2.7 x 10(4) and 8.2 x 10(3) l mol(-1)cm(-1) for method A and method B, respectively, and the corresponding Sandell sensitivity values are 0.0098 and 0.033 mu g cm(-2). The limits of detection and quantification have also been reported for both spectrophotometric methods. Intra-day and inter-day precision, and accuracy of the methods have been evaluated. The methods were successfully applied to the assay of ZDV in dosage forms and the results were statistically compared with those of the reference method by applying Student's t-test and F-test. No interference was observed from the common tablet excipients. The accuracy of the methods was further ascertained by performing recovery studies via standard addition method.

Item Type: Article
Uncontrolled Keywords: zidovudine; titrimetry; spectrophotometry; N-bromosuccinimide; pharmaceuticals
Subjects: Physical Sciences > Chemistry
Divisions: PG Campuses > Manasagangotri, Mysore > Chemistry
Depositing User: Users 7 not found.
Date Deposited: 04 Apr 2013 09:34
Last Modified: 23 Jul 2013 06:00

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