Aspergillus flavus infection and aflatoxin contamination in sorghum seeds and their biological management

Divakara, S. T. and Aiyaz, M. and Hariprasad, P. and Nayaka, S. C. and Niranjana, S. R. (2014) Aspergillus flavus infection and aflatoxin contamination in sorghum seeds and their biological management. Archives of Phytopathology and Plant Protection, 47 (17). pp. 2141-2156. ISSN 1477-2906

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Official URL: https://doi.org/10.1080/03235408.2013.869892

Abstract

In order to establish the current scenario of aflatoxigenic fungal infection and aflatoxin contamination in sorghum seeds across India, 58 seed samples were collected from different agro-climatic regions. Among these, 67.2% samples were infected with Aspergillus spp. and 28% were found contaminated with aflatoxins ranging from 0.0 to 130 μg kg-1. Greenhouse studies revealed no correlation between incidence of Aspergillus flavus and aflatoxin content, and its effect on seed quality parameters. Among the 37 A. flavus strains isolated, six were non-aflatoxigenic when analysed through cultural, TLC and ic-ELISA. Seed treatment with biocontrol agents (antagonistic Rhizobacteria and Trichoderma) suppressed the growth of A. flavus under laboratory and significantly enhanced seed quality variables under greenhouse conditions to a various extent. Field trials with selected biocontrol agents showed that talcum powder formulations of Pseudomonas putida Has-1/c, Bacillus spp. 3/a, Trichoderma asperellum M5 and T. asperellum T2 improved seedling emergence, % nutrient accumulation in plants, increased plant biomass and 1000 seed weight. Seeds harvested from treated plants showed significant increase in seed quality variables under laboratory and greenhouse conditions in comparison with control, but there was no significant difference in A. flavus infection and aflatoxin was completely absent in all treatments.

Item Type: Article
Uncontrolled Keywords: Aspergillus, Aspergillus flavus, Trichoderma asperellum, Trichoderma, Rhizobiales, Pseudomonas putida
Subjects: B Life Science > Biotechnology
Divisions: Department of > Biotechnology
Depositing User: Arshiya Kousar
Date Deposited: 18 Jun 2019 11:14
Last Modified: 18 Jun 2019 11:14
URI: http://eprints.uni-mysore.ac.in/id/eprint/3335

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