Antioxidant and neuroprotective activities of Hyptis suaveolens (L.) Poit. against oxidative stress-induced neurotoxicity

Ghaffari, H. and Ghassam, B. J. and Chandra Nayaka, S. and Ramachandra Kini, K. and Prakash, H. S. (2014) Antioxidant and neuroprotective activities of Hyptis suaveolens (L.) Poit. against oxidative stress-induced neurotoxicity. Cellular and Molecular Neurobiology, 34 (3). pp. 323-331. ISSN 0272-4340

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Official URL: https://doi.org/10.1007/s10571-013-0016-7

Abstract

The present study was carried out to investigate the antioxidant and neuroprotective effects of Hyptis suaveolens methanol extract (HSME) using various in vitro systems. The total phenol and flavonoids contents of the HSME were quantified by colorimetric methods. The HSME extract exhibited potent antioxidant activity as determined by 2,20-azino-bis(3-ethylbenzothiazoline-6- sulfonic acid) diammonium salt, 2,2-diphenyl-1-picrylhydrazyl, and ferric reducing antioxidant power assays. The neuroprotective activity of HSME was determined on mouse N2A neuroblastoma cells using 3-(4,5-dimethylthiazol-2-yl)- 2,5-diphenyltetrazolium bromide, lactate dehydrogenase, intracellular ROS assays, and upregulation of brain neuronal markers at genetic level. The N2A cells were pretreated with different concentrations (0.5, 1, 1.5, and 2 mg/ml) of the extract and then exposed to H2O2 to induce oxidative stress and neurotoxicity. The survival of the cells treated with different concentrations of HSME and H2O2 increased as compared to cells exposed only to H2O2 (47.3 %) (p < 0.05). The HSME also dose-dependently reduced LDH leakage and intracellular ROS production (p < 0.05). Pretreatment with HSME promotes the upregulation of tyrosine hydroxylase (2.41-fold, p < 0.05), and brain-derived neurotrophic factor genes (2.15-fold, p < 0.05) against H2O2-induced cytotoxicity in N2A cells. Moreover, the HSME showed antioxidant activity and decreased neurotoxicity. These observations suggest that HSME have marked antioxidant and neuroprotective activities.

Item Type: Article
Uncontrolled Keywords: controlled study, in vitro study, mouse, nonhuman, unclassified drug, animal, Animals, Cell Line, cytotoxicity, Tumor, tumor cell line, animal cell, dose response, Dose-Response Relationship, Drug, metabolism, cell survival, Cell Survival, priority journal, antioxidant, antioxidant activity, Antioxidants, physiology, plant, hydrogen peroxide, plant extract, article, phenol, MTT assay, reactive oxygen metabolite, Reactive Oxygen Species, gallic acid, oxidative stress, Oxidative Stress, isolation and purification, methanol, Plant Extracts, tyrosine 3 monooxygenase, lactate dehydrogenase, neuroprotection, neuroprotective agent, Neuroprotective Agents, Mice, colorimetry, drug effects, ferric reducing antioxidant power assay, ABTS radical scavenging assay, DPPH radical scavenging assay, upregulation, free radical scavenging assay, brain derived neurotrophic factor, neurotoxicity, Hyptis, Hyptis suaveolens, quercetin, Hyptis suaveolens extract, neuroblastoma cell, Aerial, Plant Components
Subjects: B Life Science > Biotechnology
Divisions: Department of > Biotechnology
Depositing User: Arshiya Kousar
Date Deposited: 19 Jun 2019 10:43
Last Modified: 19 Jun 2019 10:43
URI: http://eprints.uni-mysore.ac.in/id/eprint/3327

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