Rosmarinic acid mediated neuroprotective effects against H2O2-induced neuronal cell damage in N2A cells

Ghaffari, Hadi and Venkataramana, M. and Jalali Ghassam, B. and Chandra Nayaka, S. and Nataraju, A. and Geetha, N. P. and Prakash, H. S. (2014) Rosmarinic acid mediated neuroprotective effects against H2O2-induced neuronal cell damage in N2A cells. Life Sciences, 113 (1-2). pp. 7-13. ISSN 0024-3205

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Aims Oxidative stress plays a key role in several ailments including neurodegenerative conditions. The aim of the study was to demonstrate the effect of rosmarinic acid (RA) in preventing oxidative stress related death of neuronal cell lines. Main methods In the present study, we demonstrated direct neuroprotective effect of RA using H2O2-induced oxidative challenge in N2A mouse neuroblastoma cells. The mechanism of neutralization of H2O2-induced toxicity by RA was evaluated using MTT, lactate dehydrogenase, mitochondrial membrane potential (MMP), intracellular ROS, and comet assays. Up-regulation of brain neuronal markers at molecular level was performed by RT-PCR. Key findings Results presented in the paper indicate that H2O2-induced cytotoxicity in N2A cells was suppressed by treatment with RA. Moreover, RA is very effective in attenuating the disruption of lactate dehydrogenase, mitochondrial membrane potential and intracellular ROS. Pretreatment with RA significantly prevents genotoxicity (3.7-fold, p < 0.01) and promotes the up-regulation of tyrosine hydroxylase (TH) (4.5-fold, p < 0.01), and brain-derived neurotrophic factor (BDNF) genes (5.4-fold, p < 0.01) against H2O2-induced cytotoxicity in N2A cells. Significance Our results revealed that N2A cells are suitable cellular models to evaluate neuroprotective effects of RA, and suggest that RA may potentially serve as an agent for prevention of several human neurodegenerative diseases caused by oxidative stress.

Item Type: Article
Uncontrolled Keywords: Article, cell viability, controlled study, mouse, nonhuman, animal, Animals, Cell Line, chemistry, cytotoxicity, Tumor, tumor cell line, animal cell, metabolism, antioxidant, Antioxidants, gene expression, hydrogen peroxide, reactive oxygen metabolite, Reactive Oxygen Species, Mitochondrial, oxidative stress, Oxidative Stress, reverse transcription polymerase chain reaction, tyrosine 3 monooxygenase, lactate dehydrogenase, brain, Brain, neuroprotection, neuroprotective agent, Neuroprotective Agents, Mice, drug effects, cell metabolism, thiazole derivative, Thiazoles, upregulation, brain derived neurotrophic factor, genotoxicity, Hydrogen Peroxide, Tetrazolium Salts, Brain-Derived Neurotrophic Factor, Cinnamates, cinnamic acid derivative, comet assay, Comet Assay, depside, Depsides, Membrane Potential, mitochondrial membrane potential, nerve cell, nerve cell lesion, neuroblastoma cell line, Neurons, premedication, rosmarinic acid, tetrazolium, thiazolyl blue, Tyrosine 3-Monooxygenase
Subjects: B Life Science > Biotechnology
Divisions: Department of > Biotechnology
Depositing User: Arshiya Kousar Library Assistant
Date Deposited: 20 Jun 2019 06:20
Last Modified: 25 Jul 2022 06:51

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