Application of bioassay guided fractionation coupled with a molecular approach for the dereplication of antimicrobial metabolites

Rakshith, D. and Santosh, P. and Pradeep, T. P. and Gurudatt, D. M. and Syed, Baker and Yashavantha Rao, H. C. and Pasha, Azmath and Satish, S. (2016) Application of bioassay guided fractionation coupled with a molecular approach for the dereplication of antimicrobial metabolites. Chromatographia, 79 (23-24). pp. 1625-1642. ISSN 1612-1112

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A systematically delineated dereplication approach was described based on genome mining and bioassay-guided fractionation using endophytic fungus Xylaria psidii FPL-52(S) isolated from leaves of Ficus pumila Linn., (Moraceae). A polyketide synthase gene-based molecular screening strategy by a degenerate oligonucleotide primer polymerase chain reaction technique coupled with a bioinformatic phylogenomic approach revealed the presence of an iterative polyketide synthase gene within the genome of Xylaria psidii FPL-52(S). Chemical dereplication of ethyl acetate extract derived from a submerged fermentation culture broth of Xylaria psidii FPL-52(S) by bioassay-guided chromatographic and hyphenated analytical spectroscopic techniques led to the identification of polyketide mycoalexin 3-O-methylmellein. Antimicrobial profiling and minimal inhibitory concentration values for 3-O-methylmellein were determined by disc diffusion and microbroth dilution techniques. Gram-positive bacteria, dermatophytic and phytopathogenic fungi were susceptible in terms of inhibition zone and minimum inhibitory concentration values when compared to co-assayed standards. Herein, we highlight and demonstrate an improved approach which facilitates efficient dereplication and effect-guided fractionation of antimicrobial metabolite(s). The present work flow serves as a promising dereplication tool to survey the biosynthetic potential of endophytic fungal diversity, thereby identifying the most promising strains and prioritizing them for novel polyketide-derived antimicrobial metabolite discovery.

Item Type: Article
Subjects: B Life Science > Microbiology
Divisions: Department of > Microbiology
Depositing User: Manjula P Library Assistant
Date Deposited: 18 Jun 2019 06:30
Last Modified: 05 Jul 2019 09:19

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