Highly sensitive and validated spectrophotometric technique for the assay of some antidepressant drugs

Deepakumari, H. N. and Prashanth, M. K. and Vasath Kumar, B. C. and Revanasiddappa, H. D. (2015) Highly sensitive and validated spectrophotometric technique for the assay of some antidepressant drugs. JOURNAL OF APPLIED SPECTROSCOPY, 81 (6). pp. 1004-1011. ISSN 1573-8647

Full text not available from this repository. (Request a copy)
Official URL: http://doi.org/10.1007/s10812-015-0042-y

Abstract

The present paper describes a simple, rapid, reproducible, and highly sensitive spectrophotometric method for the determination of the tricyclic antidepressant drugs: amitriptyline hydrochloride (AMT), imipramine hydrochloride (IMH), clomipramine hydrochloride (CPH) and desipramine hydrochloride (DPH) in pure and in pharmaceutical preparations. The method is based on the bromination of the above drugs with known excess of bromine. The unreacted bromine is determined based on its ability to bleach the dye methyl red quantitatively at 520 nm. Regression analysis of Beer-Lambert plots showed a good correlation in the concentration range 0.0-2.5, 0-1.4, 0-1.4, and 0-1.0 mu g/ml for AMT, IMH, CPH, and DPH, respectively. The molar absorptivity values were found to be 0.65 x 10(5), 1.41 x 10(5), 1.93 x 10(5), and 2.96 x 10(5)l/mol/cm, with the corresponding Sandell's sensitivity values were 0.0048, 0.0022, 0.0018, and 0.0010 mu g/cm2 for AMT, IMH, CPH, and DPH, respectively. The limits of detection (LOD) and quantification (LOQ) are also reported for the developed method. Intra- and inter-day accuracy and precision was established according to the current ICH guidelines. Application of the procedure to the analysis of various pharmaceutical preparations gave reproducible and accurate results. Further, the validity of the proposed method was confirmed by applying the standard addition technique, and the results obtained are in good agreement with those obtained by the official method.

Item Type: Article
Subjects: C Chemical Science > Chemistry
Divisions: Department of > Chemistry
Depositing User: Shrirekha N
Date Deposited: 19 Jun 2019 05:19
Last Modified: 24 Dec 2019 07:20
URI: http://eprints.uni-mysore.ac.in/id/eprint/3268

Actions (login required)

View Item View Item