Characterization of non-aflatoxigenic strains of Aspergillus flavus as potential biocontrol agent for the management of aflatoxin contamination in groundnut

Navya, H. M. and Naveen, J. and Hariprasad, P. and Niranjana, S. R. (2017) Characterization of non-aflatoxigenic strains of Aspergillus flavus as potential biocontrol agent for the management of aflatoxin contamination in groundnut. Microbial Pathogenesis, 102. 21 - 28. ISSN 1096-1208

Full text not available from this repository. (Request a copy)
Official URL: https://doi.org/10.1016/j.micpath.2016.11.007

Abstract

In the present study, nine non-aflatoxigenic Aspergillus flavus strains were evaluated for their potential to reduce A. flavus infection and aflatoxin contamination in groundnut. Genetic characterization of these strains revealed six different deletion patterns (A-F) for thirteen examined genes from the aflatoxin biosynthesis pathway. Strain AFGS5 recorded maximum number of gene deletion (F) which included 12 out of 13 tested genes. Our findings indicated that aflR was the most frequently absent gene among the observed deletion patterns. A dendrogram inferred from combining random amplified polymorphic DNA and microsatellite data showed three of the non-aflatoxigenic strains segregating from other sampled isolates (aflatoxigenic and non-aflatoxigenic) tested. Greenhouse experiments, involving non-aflatoxigenic strains as biocontrol agents resulted in two strains, AFGS5 and AFGS12, which significantly reduced the population of aflatoxigenic fungi and the level of total aflatoxins in the rhizosphere/geocarposphere of soil samples as well as in groundnut seeds. Based on our findings, the use of these native non-aflatoxigenic strains; AFGS5 or AFGS12 in particular, as biopesticides could offer efficacious mitigation of aflatoxin contamination.

Item Type: Article
Uncontrolled Keywords: PCR, Deletion pattern, Rhizosphere, Biopesticides
Subjects: B Life Science > Biotechnology
Divisions: Department of > Biotechnology
Depositing User: MUL SWAPNA user
Date Deposited: 17 Jun 2019 04:28
Last Modified: 17 Jun 2019 04:28
URI: http://eprints.uni-mysore.ac.in/id/eprint/3199

Actions (login required)

View Item View Item