Anti-inflammatory and antinociceptive effect of pachygone ovataleaves

Maraheland, Shirin and Umesha, S. (2016) Anti-inflammatory and antinociceptive effect of pachygone ovataleaves. Harmaceutical biology, 54 (12). pp. 3046-3054.

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Official URL: http://dx.doi.org/10.1080/13880209.2016.1203960

Abstract

Context:Pachygone ovata(Poir.) Miers ex Hook. F. et Thoms (Menispermaceae) is a rich source of bioactive bisbenzylisoquinoline and aporphine alkaloids.Objective:This study investigates thein vitroandin vivoanti-inflammatory and antino ciceptive potential of Pachygone ovataleaves.Materials and methods:Lipoxygenase (LOX) assay for anti-inflammatory activity was conducted usingMeOH, EA, H and Aq extracts; followed by alkaloid isolation. The anti-inflammatory potential was deter-mined using carrageenan-induced paw oedema and formalin tests for evaluation ofPachygone ovataanal-gesic effect. Different doses (100, 300 and 400lg/kg) were administered orally to Wistar rats for a periodof one week, once daily.Results:MeOH and EA extract efficiently inhibited LOX (IC501.43 and 2.15lg/mL, respectively). MeOHextract had better inhibiting capacity (57%) than indomethacin (51%) in carrageenan induced rats. MeOHextract (300lg/kg) significantly reduced the increased levels of nitric oxide (8 ± 0.57 M), total leukocytecount (4.5 ± 0.05 cells 103/cells) and C-reactive protein (55 ± 0.45 mg/mL). There was a decrease in variousserum biochemical markers (ALT, AST). Histopathological studies revealed reduction in oedema anddecreased cellular infiltration on supplementation with MeOH extract. Furthermore, MeOH extract (300lg/kg)and alkaloid fraction (400lg/kg) effected both phases (neurogenic and inflammatory) of formalin injectedmodels.Discussion and conclusion:Inflammatory mediators play a key role in inflammation; therefore, keeping itin control is of utmost importance. The usefulness ofPachygone ovataleaves on pain and inflammationhas been described, probably due to its effect on inflammatory mediators and high alkaloid content.

Item Type: Article
Subjects: B Life Science > Biotechnology
Divisions: Department of > Biotechnology
Depositing User: manjula User
Date Deposited: 15 Jun 2019 10:23
Last Modified: 18 Sep 2019 11:36
URI: http://eprints.uni-mysore.ac.in/id/eprint/3150

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