Attachment and biofilm formation of Pseudomonas fluorescens PSD4 isolated from a dairy processing line

Aswathanarayan, J. B. and Rai, V. Ravishankar (2014) Attachment and biofilm formation of Pseudomonas fluorescens PSD4 isolated from a dairy processing line. Food Science and Biotechnology, 23 (6). pp. 1903-1910. ISSN 2092-6456

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Official URL: https://doi.org/10.1007/s10068-014-0260-8

Abstract

The effects of different nutrient sources and temperatures on attachment and biofilm formation of Pseudomonas fluorescens PSD4, a dairy isolate, were studied. Initial adherence and attachment capabilities among different strains were studied using microtitre plate assays. Biofilm development was observed using confocal microscopy. Strongly adherent cells were seen in protein rich media. Citrate as a carbon source enhanced biofilm formation. Glucose did not favor biofilm development. Psychrotrophic P. fluorescens PSD4 formed strongly adherent biofilms having high metabolic activities at low temperatures. P. fluorescens PSD4 with spoilage potential was capable of forming strong biofilms in dairy processing environments. Biofilm formation was influenced by nutrient availability and growth conditions. These factors should be considered for design of effective anti-biofilm strategies. © 2014, The Korean Society of Food Science and Technology and Springer Science+Business Media Dordrecht.

Item Type: Article
Uncontrolled Keywords: Bacteria, Biofilms, Pseudomonas fluorescens, Food safety, Biofilm development, Biofilm formation, Dairy processing, Dairy products, Growth conditions, Metabolic activity, Nutrient availability, Nutrient sources, Nutrients
Subjects: B Life Science > Microbiology
Divisions: Department of > Microbiology
Depositing User: Arshiya Kousar
Date Deposited: 14 Jun 2019 10:06
Last Modified: 22 Dec 2020 05:15
URI: http://eprints.uni-mysore.ac.in/id/eprint/3066

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