Nested PCR method for early detection of fumonisin producing fusarium verticillioides in pure cultures, cereal samples and plant parts

Deepa, N. and Adkar-Purushothama, Charith Raj and Sreenivasa, M. Y. (2016) Nested PCR method for early detection of fumonisin producing fusarium verticillioides in pure cultures, cereal samples and plant parts. Food Biotechnology, 30 (1). pp. 18-29. ISSN 1532-424

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Official URL: https://doi.org/10.1080/08905436.2015.1129502

Abstract

Fumonisin-producing Fusarium verticillioides was detected in cereal samples by semi-nested PCR using one forward, VERTF-1, and two reverse primers, VERTR and VERTF-2. A total of 326 Fusarium species isolated from maize, paddy, sorghum, wheat and pearl-millet samples were subjected to a first round of n-PCR with the species specific VERTF-1 and VERTR set of primers which recorded 59.50% of F. verticillioides. Further, second round of n-PCR scored 53.98% of fumonisin-producing F. verticillioides with fumonisin specific VERTF-1 and VERTF-2 set of primers. Maize samples recorded the highest frequency of fumonisin-producing F. verticillioides 40.98%, followed by paddy 33.33%, and sorghum 12.50%. Sensitivity of nested PCR was conducted by whole grain experiment of the cereals, roots and leaves of the cereals by diluting the DNA 10-100 times, in which 1:50, 1:75, and 1:100 diluted samples recorded positive. This method can be used for the early detection of fumonisin producing F. verticillioides occurring on cereals

Item Type: Article
Subjects: B Life Science > Microbiology
Divisions: Department of > Microbiology
Depositing User: manjula User
Date Deposited: 13 Jun 2019 05:21
Last Modified: 13 Jun 2019 05:21
URI: http://eprints.uni-mysore.ac.in/id/eprint/3000

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