Streptomycete endophytes from anti-diabetic medicinal plants of the Western Ghats inhibit alpha-amylase and promote glucose uptake

Akshatha, V. J. and Nalini, M. S. and D'Souza, C. and Prakash, H. S. (2014) Streptomycete endophytes from anti-diabetic medicinal plants of the Western Ghats inhibit alpha-amylase and promote glucose uptake. Letters in Applied Microbiology, 58 (5). pp. 433-439. ISSN 0266-8254

[img] Text (Full Text)
Bot_2014_Akshatha.pdf - Published Version
Restricted to Registered users only

Download (416kB) | Request a copy
Official URL:


α‐amylase inhibitor retards the liberation of glucose from dietary complex carbohydrates and delays the absorption of glucose. The purpose of the study was to isolate and select α‐amylase inhibitor‐producing endophytic actinomycetes from the leaves and stems of Leucas ciliata and Rauwolfia densiflora, two of the well‐known medicinal plants used in the treatment for diabetes. Sterilized plant samples were inoculated on the actinomycete isolation agar medium containing 50 ppm cycloheximide and incubated for 4–8 weeks at room temperature. The actinomycetes were isolated on agar medium and identified on the basis of 16S rRNA sequences, the isolates exhibiting >99% similarities were submitted to NCBI, and gene accession numbers were obtained. They were inoculated to International Streptomyces Project 1 medium (ISP 1) for fermentation. The extracts obtained were tested for the anti‐diabetic potential by the inhibition of alpha‐amylase by colorimetric assay and glucose uptake in the porcine hemidiaphragm. Streptomyces longisporoflavus (JX965948) isolated from the stem fragments of L. ciliata exhibited alpha‐amylase inhibitory activity (IC50 values = 162·3 ± 1·05 μg ml−1) in comparison with the standard Acarbose™ (IC50 value = 73·1 ± 1·12 μg ml−1). Extract of Streptomyces sp. (JQ926174) from R. densiflora indicated glucose uptake in the porcine hemidiaphragm. Results indicate for the first time the potential of endophytic streptomycete extracts with anti‐diabetic activity.

Item Type: Article
Uncontrolled Keywords: controlled study, human, unclassified drug, Animals, antibiotic agent, nucleotide sequence, RNA sequence, glucose, India, amylase, enzyme inhibition, article, antidiabetic activity, glucose transport, room temperature, medicinal plant, bacterium, fermentation, Western Ghats, Hypoglycemic Agents, Plant Extracts, inoculation, Plant Leaves, amylase inhibitor, Actinobacteria, RNA 16S, Streptomyces, colorimetry, endophyte, Endophytes, Streptomyces sp., IC 50, Plants, Medicinal, diabetes, inhibitor, biodiversity, Molecular Sequence Data, biotechnology, standard, inhibition, acarbose, Biological Assay, identification method, disease control, swine, Actinobacteria (class), agar medium, alpha-Amylases, biological uptake, cycloheximide, endophytic actinomycetes, germplasm, glucose uptake, hemidiaphragm, leaf, Leucas, Leucas ciliata, medicinal plants, medicinal species, molecular analysis, molecular characterization, Plant Stems, Rauvolfia densiflora, Rauwolfia densiflora, stem, Streptomyces longisporoflavus, Streptomyces longisporoflavus extract, Sus, Swine, α-amylase inhibition
Subjects: B Life Science > Biotechnology
B Life Science > Botany
C Chemical Science > Biochemistry
Divisions: Department of > Biochemistry
Department of > Biotechnology
Department of > Botany
Depositing User: Arshiya Kousar Library Assistant
Date Deposited: 13 Jun 2019 10:54
Last Modified: 09 Jul 2019 11:28

Actions (login required)

View Item View Item