Analysis of dynamics of proteome in resistant cultivar of pearl millet seedlings during sclerospora graminicola infection

Anup, Chandra Pal and Kini, K. R. (2016) Analysis of dynamics of proteome in resistant cultivar of pearl millet seedlings during sclerospora graminicola infection. Journal of Applied Biology & Biotechnology, 4 (2). 067-071. ISSN 2347-212X

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Official URL: https://doi.org/10.7324/JABB.2016.40210

Abstract

The current study was performed to analyze the dynamics of proteome in resistant cultivar of pearl millet seedlings during early hours of downy mildew infection. This was achieved using two-dimensional gel electrophoresis (2DE)-nano LCMS/MS based approach. Total protein extract from two-day-old pearl millet seedlings harvested 6 h post inoculation with pathogen, was fractionated by 2DE on 5-8 pI range IPG strips. Among the 210 protein spots detected by the PD-Quest software, 21 proteins were found to be up-regulated and 14 proteins down-regulated. Differential regulation of these proteins during downy mildew infection was found to be significant at p ≤ 0.05. Majority of these proteins belonged to the following functional categories: energy and metabolism (32) category as well as stress and defense (32). Unlike the previous proteomic study in pearl millet, the present study showed differential accumulation of early expressing defense-related genes such as those involved in reactive oxygen species mediated pathways, pathogenesis-related proteins and other interconnecting pathways responsible for coordinating the resistance mechanism of the plant. The current findings shed light on the vital differentially regulated proteins of pearl millet during early phase of pathogen infection that can be targeted as future disease management strategy.

Item Type: Article
Uncontrolled Keywords: Pearl millet,downy mildew,early expressing genes,differential regulation,proteomics.
Subjects: B Life Science > Biotechnology
Divisions: Department of > Biotechnology
Depositing User: manjula User
Date Deposited: 10 Jun 2019 07:38
Last Modified: 01 Jul 2019 11:27
URI: http://eprints.uni-mysore.ac.in/id/eprint/2896

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