Detection of fumonisin producing Fusarium verticillioides in paddy (Oryza sativa L.) using polymerase chain reaction (PCR)

Maheshwar, P. K. and Moharram, S. Ahmed and Janardhana, G. R. (2009) Detection of fumonisin producing Fusarium verticillioides in paddy (Oryza sativa L.) using polymerase chain reaction (PCR). BRAZILIAN JOURNAL OF MICROBIOLOGY, 40 (1). pp. 134-138.

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Official URL: http://dx.doi.org/10.1590/S1517-83822009000100023

Abstract

The study reports the occurrence of fumonisin producing Fusarium verticillioides in 90 samples of stored paddy (Oryza sativa L.) collected from different geographical regions of Karnataka, India. Fumonisin producing F. verticillioides was identified based on micromorphological characteristics and PCR using two sets of primers. One set of primers was F. verticillioides species specific, which selectively amplified the intergenic space region of rDNA. The other set of primers was specific to fumonisin producing F. verticillioides. Eight paddy samples were positive for F. verticillioides. Eleven isolates obtained from these samples were capable of producing fumonisin.

Item Type: Article
Uncontrolled Keywords: Fusarium verticillioides; fumonisin; paddy; PCR
Subjects: Agriculture Biological Sciences > Botany
University of Mysore > PG Campuses > Manasagangotri, Mysore > Agriculture Biological Sciences > Botany
Divisions: PG Campuses > Manasagangotri, Mysore > Botany
Depositing User: Users 1 not found.
Date Deposited: 02 Apr 2013 10:51
Last Modified: 25 Sep 2013 11:32
URI: http://eprints.uni-mysore.ac.in/id/eprint/2836

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