Genetic diversity among `Candidatus Liberibacter asiaticus' isolates based on single nucleotide polymorphisms in 16S rRNA and ribosomal protein genes

Adkar-Purushothama, C. R. and Fabio Quaglino, and Paola Casati, and Ramanayaka, J. G. and Piero Attilio Bianco, (2009) Genetic diversity among `Candidatus Liberibacter asiaticus' isolates based on single nucleotide polymorphisms in 16S rRNA and ribosomal protein genes. ANNALS OF MICROBIOLOGY, 59 (4). pp. 681-688.

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Official URL: http://dx.doi.org/10.1007/BF03179208

Abstract

Citrus Huanglongbing (HLB) is one of the most destructive and widespread diseases of citrus, caused by `Candidatus Liberibacter', a non-cultured, phloem-restricted alpha-proteobacterium. In the present study, `Ca. Liberibacter' was detected in symptomatic citrus plants growing in the Karnataka state (South India) by amplification of 16S rRNA and P-operon ribosomal protein (beta-rp) genes, using PCR primers specific for African and Asian `Ca. Liberibacter'. Sequencing of the 16S rRNA amplified fragment revealed that `Ca. Liberibacter', here identified belongs to the species `Ca. Liberibacter asiaticus'. The alignments of 16S rRNA and ribosomal protein gene sequences of all known `Ca. Liberibacter asiaticus'-related, allowed to recognize Single Nucleotide Polymorphisms (SNPs). Basing on SNP analysis, the strains were grouped in fourteen 16SrRNA SNP genetic lineages (16Sr-I to 16Sr-XIV) and three beta-rp SNP genetic lineages (rp-I to rp-III). Only the strains of `Ca. Liberibacter asiaticus' from the Karnataka state belonged to the genetic lineages 16Sr-I and rp-I. RFLP diagnostic tests on the discriminative 16S rRNA SNPs were set up to identify this lineage. These results revealed the presence of a new `Ca. Liberibacter asiaticus' genetic lineage in the Indian sub-continent, where at least four genetically diverse SNP lineages were found. These findings could open new opportunities for in-depth studies on biological niches and traits of `Ca. Liberibacter asiaticus'.

Item Type: Article
Uncontrolled Keywords: Huanglongbing (HLB); `Candidatus Liberibacter'; single nucleotide polymorphisms; genetic lineage
Subjects: Agriculture Biological Sciences > Botany
University of Mysore > PG Campuses > Manasagangotri, Mysore > Agriculture Biological Sciences > Botany
Divisions: PG Campuses > Manasagangotri, Mysore > Botany
Depositing User: Users 1 not found.
Date Deposited: 02 Apr 2013 10:21
Last Modified: 07 Sep 2015 06:54
URI: http://eprints.uni-mysore.ac.in/id/eprint/2828

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