Detection, identification and phylogenetic analysis of indian ralstonia solanacearum isolates by comparison of partial hrpb gene sequences

Chandrasekhar, B. and Umesha, S. (2015) Detection, identification and phylogenetic analysis of indian ralstonia solanacearum isolates by comparison of partial hrpb gene sequences. JOURNAL OF PHYTOPATHOLOGY, 163 (2). pp. 105-115. ISSN 1439-0434

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Official URL: https://doi.org/10.1111/jph.12287

Abstract

A species-specific Polymerase Chain Reaction (sPCR) method was developed to identify and detect isolates of Ralstonia solanacearum, the cause of bacterial wilt disease in chilli. PCR primers for R.solanacearum were identified by alignment of hrpB gene sequences and selection of sequences specific for R.solanacearum at their 3 ends. The primers were shown to be specific for R.solanacearum, as no PCR product was obtained when genomic DNA from other bacterial species including closely related Ralstonia species, were used as test species. Lone pair of primers (RshrpBF and RshrpBR) was designed using hrpB gene sequence, unique to R.solanacearum which amplified a predicted PCR product of 810bp from 20 different isolates. Phylogenetic analysis was also attempted to understand the evolutionary divergence of Indian R.solanacearum isolates. Based on phylogenetic analysis, Indian isolates showed homology with the standard reference isolates from other countries but, interestingly, one new isolate showed complete evolutionary divergence by forming an out-group.

Item Type: Article
Subjects: B Life Science > Biotechnology
Divisions: Department of > Biotechnology
Depositing User: Shrirekha N
Date Deposited: 17 Jun 2019 05:09
Last Modified: 17 Jun 2019 05:09
URI: http://eprints.uni-mysore.ac.in/id/eprint/2800

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