Antidiabetic and antioxidant potency evaluation of different fractions obtained from Cucumis prophetarum fruit

Gawli, K. and Lakshmidevi, N. (2015) Antidiabetic and antioxidant potency evaluation of different fractions obtained from Cucumis prophetarum fruit. PHARMACEUTICAL BIOLOGY, 53 (5). pp. 689-694. ISSN 1744-5116

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Official URL: https://doi.org/10.3109/13880209.2014.937503

Abstract

Context: Cucumis prophetarum Linn. (Cucurbitaceae) fruit is used for inflammatory-related problems and is proved to be possessing anticancer and hepatoprotective effects. Objective: The present investigation was to study the effect of different fractions of Cucumis prophetarum on antidiabetic and antioxidant activity. Materials and methods: Aqueous crude extract (CE) of Cucumis prophetarum fruits was fractionated into water soluble fraction 1 (F1), chloroform fraction 2 (F2), basic fraction 3 (F3), and neutral fraction 4 (F4) by acid-base extraction. CE and its fractions at different doses (0.02-0.1 mg/mL) were subjected to antidiabetic (alpha-amylase and alpha-glucosidase inhibition assays) and antioxidant (DPPH, superoxide radical scavenging (SO) and metal chelation) evaluation. Results: F1 exhibited effective antidiabetic activity (p < 0.05) with an IC50 value of 20.6 and 59.9 mu g/mL. The activity decreased in the order of CE > F4 > F3 > F2, according to alpha-amylase assay, which were the same, with the exception of the rank order of F4 and CE, as the alpha-glucosidase assay. Furthermore, F1 (IC50 = 73 mu g/mL) showed better reducing ability than CE > F4 > F2 > F3 (IC50 = 78-272 mu g/mL), according to the DPPH assay. In SO and metal chelation assays, F1 showed the highest activity (IC50 = 101 and 147 mg/mL), respectively; the activity decreased in the order of CE > F4 > F3 > F2 (IC50 = 126-469 mu g/mL) for SO and 194-944 mu g/mL for metal chelation assay. Conclusion: The results indicate that F1 possesses potent in vitro antidiabetic and antioxidant activities.

Item Type: Article
Subjects: B Life Science > Microbiology
Divisions: Department of > Microbiology
Depositing User: Shrirekha N
Date Deposited: 11 Jun 2019 06:34
Last Modified: 11 Jun 2019 06:34
URI: http://eprints.uni-mysore.ac.in/id/eprint/2739

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