Determination of raloxifene hydrochloride in human urine by LC-MS-MS

Anil Kumar, U. R. and Basavaiah, K. and Tharpa, K. and Vinay, K. B. (2009) Determination of raloxifene hydrochloride in human urine by LC-MS-MS. CHEMICAL INDUSTRY & CHEMICAL ENGINEERING QUARTERLY, 15 (3). pp. 119-123.

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Abstract

A sensitive and selective liquid chromatographic-tandem mass spectrometric (LC-MS-MS) method was developed to determine raloxifene hydrochloride (RLX) in human urine. After a solid-phase extraction with SPE cartridge, the urine sample was analyzed on a C(18) column (Symmetry 3.5 mu m; 50 mmx4.6 mm id) interfaced with a triple quadruple tandem mass spectrometer. A positive electrospray Ionization was employed as the Ionization source. The mobile phase consisted of ammonium acetate (pH 4.0)-acetonitrile (60.40, v/v). The method was linear over a concentration range of 20-1000 ng mL(-1). The lower limit of quantitation was 20 ng mL(-1). The intra-day and inter-day relative standard deviation across three validation runs over the entire concentration range was <10.5%. The accuracy determined at three concentrations (50, 500 and 850 ng mL(-1) RLX) was within +/- 0.84% in terms of relative errors.

Item Type: Article
Uncontrolled Keywords: raloxifene; quantification; LC-MS-MS; urine
Subjects: Physical Sciences > Chemistry
Divisions: PG Campuses > Manasagangotri, Mysore > Chemistry
Depositing User: Users 1 not found.
Date Deposited: 01 Apr 2013 08:44
Last Modified: 25 Sep 2013 07:36
URI: http://eprints.uni-mysore.ac.in/id/eprint/2737

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