Inhibition of snake venom metalloproteinase by β-Lactoglobulin peptide from buffalo (Bubalus bubalis) colostrum

Arpitha, Ashok and Sebastin, M. Santhosh and Rohit, A. C. and Girish, K. S. and Vinod, D. and Aparna, H. S. (2017) Inhibition of snake venom metalloproteinase by β-Lactoglobulin peptide from buffalo (Bubalus bubalis) colostrum. Applied Biochemistry and Biotechnology, 182 (4). pp. 1415-1432. ISSN 1559-0291

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Official URL: https://doi.org/10.1007/s12010-017-2407-6

Abstract

Bioactive peptide research has experienced considerable therapeutic interest owing to varied physiological functions, efficacy in excretion, and tolerability of peptides. Colostrum is a rich natural source of bioactive peptides with many properties elucidated such as anti-thrombotic, anti-hypertensive, opioid, immunomodulatory, etc. In this study, a variant peptide derived from β-lactoglobulin from buffalo colostrum was evaluated for the anti-ophidian property by targeting snake venom metalloproteinases. These are responsible for rapid local tissue damages that develop after snakebite such as edema, hemorrhage, myonecrosis, and extracellular matrix degradation. The peptide identified by LC-MS/MS effectively neutralized hemorrhagic activity of the Echis carinatus venom in a dose-dependent manner. Histological examinations revealed that the peptide mitigated basement membrane degradation and accumulation of inflammatory leucocytes at the venom-injected site. Inhibition of proteolytic activity was evidenced in both casein and gelatin zymograms. Also, inhibition of fibrinolytic and fibrinogenolytic activities was seen. The UV-visible spectral study implicated Zn2+ chelation, which was further confirmed by molecular docking and dynamic studies by assessing molecular interactions, thus implicating the probable mechanism for inhibition of venom-induced proteolytic and hemorrhagic activities. The present investigation establishes newer vista for the BLG-col peptide with anti-ophidian efficacy as a promising candidate for therapeutic interventions.

Item Type: Article
Uncontrolled Keywords: Colostrum, β-Lactoglobulin, Peptide, Snake venom, metalloproteinases, Molecular dynamics
Subjects: B Life Science > Biotechnology
Divisions: Department of > Biotechnology
Depositing User: MUL SWAPNA user
Date Deposited: 06 Jun 2019 06:17
Last Modified: 29 Jun 2019 05:44
URI: http://eprints.uni-mysore.ac.in/id/eprint/2662

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