Ashok, N. R. and Aparna, H. S. (2017) Empirical and bioinformatic characterization of buffalo (Bubalus bubalis) colostrum whey peptides & their angiotensin I-converting enzyme inhibition. Food Chemistry, 228. 582 - 594. ISSN 0308-8146
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Abstract
Whey based peptides are well known for their nutritional and multifunctional properties. In this context, whey proteins from buffalo colostrum & milk were digested by in vitro simulation digestion and analyzed by nano-LC–MS/MS. Functional protein association networks, gene annotations and localization of identified proteins were carried out. An ACE inhibitory peptide sorted from the library was custom synthesized and an in vitro ACE assay was performed. The study led to the identification of 74 small peptides which were clustered into 5 gene functional groups and majority of them were secretory proteins. Among the identified peptides, majority of them were found identical to angiotensin I-converting enzyme (ACE) inhibitors, antioxidant, antimicrobial, immunomodulatory and opioidal peptides. An octapeptide (m/z – 902.51, IQKVAGTW) synthesized was found to inhibit ACE with an IC50 of 300±2µM. The present investigation thus establishes newer vista for food derived peptides having ACE inhibitory potential for nutraceutical or therapeutic applications.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Buffalo colostrum, Whey peptides, Nano-LC–MS/MS, Molecular docking, Angiotensin I-converting enzyme |
| Subjects: | B Life Science > Biotechnology |
| Divisions: | Department of > Biotechnology |
| Depositing User: | C Swapna Library Assistant |
| Date Deposited: | 06 Jun 2019 06:03 |
| Last Modified: | 06 Jun 2019 06:03 |
| URI: | http://eprints.uni-mysore.ac.in/id/eprint/2658 |
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