Utilization of bromination reaction for the spectrophotometric assay of domperidone

Devi, O. Z. and Basavaiah, K. and Vinay, K. B. (2011) Utilization of bromination reaction for the spectrophotometric assay of domperidone. CHEMICAL INDUSTRY & CHEMICAL ENGINEERING QUARTERLY, 17 (1). pp. 81-89. ISSN 1451-9372

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Three simple and sensitive spectrophotometric methods are described for the determination of domperidone (DOM) in bulk drug and in dosage forms using bromate-bromide mixture as brominating agent in acid medium and three dyes, meta-cresol purple (MCP), amaranth (AMR) and erioglaucine (EGC). The methods involve the addition of a known excess of bromate-bromide mixture to an acidified solution of DOM followed by the determination of the residual bromine by reacting with a fixed amount of either MCP dye and measuring the absorbance at 530 nm (method A) or AMR dye and measuring the absorbance at 520 nm (method B) or EGC dye and measuring the absorbance at 630 nm (method C). Beer's law is obeyed over the concentration ranges, 0.63-10.0, 0.25-4.0 and 0.13-2.0 mu g mL(-1) for method A, B and C, respectively. The apparent molar absorptivities are calculated to be 3.751x10(4), 6.604x10(4) and 1.987x10(5) L mol(1) cm(-1) for method A, B and C, respectively, and the corresponding sandell sensitivity values are 0.011, 0.006 and 0.002 mu g cm(-2). The limit of detection and the limit of quantification are also reported for all the three methods. No interference was observed from common additives found in pharmaceutical preparations. Statistical comparisons of the results with those of the reference method showed excellent agreement, and indicated no significant difference in accuracy and precision. The accuracy and reliability of the methods were further ascertained by performing recovery tests via standard-addition technique.

Item Type: Article
Uncontrolled Keywords: domperidon; assay; spectrophotometry; bromate-bromide; dyes; tablets
Subjects: C Chemical Science > Chemistry
Divisions: Department of > Chemistry
Depositing User: Users 23 not found.
Date Deposited: 04 Jun 2019 13:56
Last Modified: 04 Jun 2019 13:56
URI: http://eprints.uni-mysore.ac.in/id/eprint/2601

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