Bioactivity guided isolation of mosquito larvicide from piper longum

Madhu, S. K. and Vijayan, V. A. and Shaukath Ara Khanum (2011) Bioactivity guided isolation of mosquito larvicide from piper longum. Asian Pacific Journal of Tropical Medicine, 4 (2). pp. 112-116.

[img] Text (full text)

Download (315kB)
Official URL:


Objective: To isolate the larvicidal component from the fruits of Piper Ion guns (P. Ion guns) against the filariasis vector, Culex quinquefasciatus (C. quinquefasciatus). Methods: Pulverized fruits of P. longum were subjected to soxhlet extraction using series of organic solvents of increasing polarity. All the solvent extracts were verified for their larvicidal efficacy against 4th instar larvae of C. quinquefasciatus employing standard WHO procedure. Bioassay guided fractionation through column chromatography lead to the isolation of a bioactive amide, pipyahyine from the petroleum ether extract. Results: Petroleum ether extract was found to be the most active fraction among all the extracts tested with LC(50) and LC(90) being 1.03 and 2.04 ppm respectively. Whereas, pipyahyine, an isolated component of the same fraction was found to be even more effective than the parent extract in terms of LC(50) being 0.58 and 1.88 ppm respectively. Conclusions: From the results, it is evident that P. longum can be considered as a powerful arsenal for the control of mosquito population.

Item Type: Article
Uncontrolled Keywords: Piper longum; Bioassay; Pipyahyine; Culex quinquefasciatus; Filariasis; Larvicide
Subjects: C Chemical Science > Chemistry
Divisions: Department of > Zoology
Depositing User: Users 23 not found.
Date Deposited: 20 Jun 2019 09:34
Last Modified: 24 Nov 2022 06:17

Actions (login required)

View Item View Item