Neutralization of local toxicity induced by vipera russelli phospholipase A(2) by lipophilic derivative of ascorbic acid

Mohamed, R. and Shivaprasad, H. V. and Jameel, N. M. and Shekar, M. A. and Vishwanath, B. S. (2011) Neutralization of local toxicity induced by vipera russelli phospholipase A(2) by lipophilic derivative of ascorbic acid. CURRENT TOPICS IN MEDICINAL CHEMISTRY, 11 (20). pp. 2531-2539. ISSN 1568-0266

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Official URL: DOI : 10.2174/156802611797633429


L-ascorbic acid upon condensation with palmitic acid in the presence of sulphuric acid results in L-ascorbic acid-6-palmitate (AP). The effect of L-ascorbic acid derivative, AP on the pharmacological activities of purified basic multi-toxic PLA(2) enzyme, VRV-PL-VIIIa from Vipera russelli snake venom along with in vitro activities is described. AP inhibited VRV-PL-VIIIa enzyme activity in a concentration dependent manner with IC50 value of 48.85 mu M and the inhibition is found to be independent of substrate and calcium concentration. Upon investigating the in vivo pharmacological activities, it has been found that AP inhibited VRV-PL-VIIIa induced mouse paw edematogenic activity in a dose dependant manner. Intramuscular co-injection of AP with VRV-PL-VIIIa (1:10 w:w) neutralized the VRV-PL-VIIIa induced myotoxocity. Sections of mouse thigh muscle showed normal intact musculature with normal levels of serum creatine kinase and lactate dehydrogenase. Histopathological studies showed that administration of VRV-PL-VIIIa (i.p) along with AP mixture inhibited VRV-PL-VIIIa induced lung haemorrhage in mouse indicated that enzyme activity is responsible for all these observed pathological and pharmacological activities. The biophysical interaction studies showed that AP interacted directly with the enzyme and decreased the relative intrinsic fluorescence intensity. CD spectral analysis showed an apparent shift in the far UV-CD spectra of VRV-PL-VIIIa with AP. Docking study also confirmed the interaction of AP with enzyme directly. These results demonstrate that AP neutralizes VRV-PL-VIIIa induced pharmacological activities by inhibiting the enzyme with direct interactions. This compound along with other inhibitors of snake venom hydrolytic enzymes might be of use to neutralize local toxicity of V. russelli venom where antivenoms have failed.

Item Type: Article
Uncontrolled Keywords: Ascorbic acid-6-palmitate; Vipera russelli; PLA(2); myotoxicity; lung haemorrhage
Subjects: C Chemical Science > Biochemistry
Divisions: Department of > Biochemistry
Depositing User: Users 23 not found.
Date Deposited: 16 Aug 2019 06:02
Last Modified: 16 Aug 2019 06:02

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