Effect of heat treatment on chromosomal aberrations induced by the alkylating agent trenimon or the restriction endonuclease Alu I in Chinese hamster ovary (CHO) cells

Vasudev, V. and Obe, G. (1987) Effect of heat treatment on chromosomal aberrations induced by the alkylating agent trenimon or the restriction endonuclease Alu I in Chinese hamster ovary (CHO) cells. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, 178 (1). pp. 81-90. ISSN 0027-5107

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Official URL: https://doi.org/10.1016/0027-5107(87)90089-3

Abstract

Heat treatment of CHO cells in the G1-phase of the cell cycle leads to chromatid-type aberrations in first posttreatment metaphases. Posttreatment of heat-treated cells with the alkylating agent trenimon leads to a synergistic effect on the production of chromatid-type exchanges. These results indicate that heat induces lesions which like the lesions produced by trenimon give rise to chromatid-type aberrations during the first posttreatment S-phase, and that these lesions can interact with each other to produce chromatid-type exchanges. Treatment of CHO cells in the H1-phase of the cell cycle with the restriction endonuclease Alu I induces chromosomal aberrations. Pretreatment of cells with heat leads to a reduction of Alu I induced chromosome-type aberrations. When cells are allowed to recover after heat treatment for 22 h, the aberration frequencies produced by Alu I are the same as in cells not treated with heat. These findings can be explained by assuming that heat-induced accumulation of accessory proteins in the chromatin protects the DNA from being cut by Alu I, and that the cells recovered from the heat-induced protein accumulation after 22 h.

Item Type: Article
Additional Information: Unmapped bibliographic data: DA - 1987/05/01/ [EPrints field already has value set] JO - Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis [Field not mapped to EPrints]
Uncontrolled Keywords: Heat treatment, Chromosomal aberrations, Trenimon, Restriction endonuclease Alu I, (Chinese hamster ovary cells)
Subjects: Agriculture Biological Sciences > Zoology
University of Mysore > PG Campuses > Manasagangotri, Mysore > Agriculture Biological Sciences > Zoology
Divisions: PG Campuses > Manasagangotri, Mysore > Zoology
Depositing User: Praveen Kumari B. L.
Date Deposited: 26 Mar 2018 06:08
Last Modified: 26 Mar 2018 06:08
URI: http://eprints.uni-mysore.ac.in/id/eprint/20998

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