Mamatha Bhanu, L. S. and Nishimura, S. L. and Aparna, H. S. (2016) Inhibitory potential of Buffalo (Bubalus bubalis) colostrum immunoglobulin G on Klebsiella pneumoniae. International Journal of Biological Macromolecules, 88. 138 - 145.
Full text not available from this repository. (Request a copy)Abstract
The unique components of colostrum like free oligosaccharides and glycoconjugates are known to offer resistance to enzymatic digestion in the gastrointestinal tract and have the ability to inhibit the localized adherence of enteropathogens to the digestive tract of the neonates. In this context, we have evaluated the in vitro effect of buffalo colostrum immunoglobulin G on human pathogen Klebsiella pneumoniae, a predominant multidrug resistant pathogen associated with nasocomial infections. The investigation revealed growth inhibitory potential of immunoglobulin G in a dose dependent manner supported by scanning electron microscopic studies. The N-glycan enriched fraction of immunoglobulin G after PNGase treatment was found more effective, comparable to ampicillin than native immunoglobulin G supporting the fact that colostrum derived oligosaccharides is crucial and act as ideal substrates for undesirable and pathogenic bacteria. The MALDI TOF/TOF analysis confirmed the glycostructures of abundant N-glycans of immunoglobulin G exerting antibacterial activity. The proteomic analysis revealed variations between control and treated cells and expression of chemotaxis⿿CheY protein (14kDa) was evidenced in response to immunoglobulin G treatment. Hence, it would be interesting to investigate the mode of inhibition of multidrug-resistant K. pneumoniae by buffalo colostrum immunoglobulin G with the identification of a newly expressed signalling protein.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Buffalo colostrum, Immunoglobulin G, -Glycans, , MALDI-TOF |
| Subjects: | B Life Science > Biotechnology |
| Divisions: | Department of > Biotechnology |
| Depositing User: | Manjula P Library Assistant |
| Date Deposited: | 03 Jun 2019 08:05 |
| Last Modified: | 10 Dec 2019 09:36 |
| URI: | http://eprints.uni-mysore.ac.in/id/eprint/2097 |
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