Virulence factors of E. coli ST131 and its H30 and H30Rx subclones among extended-spectrum beta-lactamase producing isolates

AL-hetar, K. Y. A. and Lakshmidevi, N. (2016) Virulence factors of E. coli ST131 and its H30 and H30Rx subclones among extended-spectrum beta-lactamase producing isolates. International Journal of Genetics and Genomics, 4 (4). pp. 36-39.

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Abstract

Escherichia coli sequence type 131 (ST131) is a pandemic clone causing predominantly community and hospital-onset antimicrobial-resistant infections. Recently, H30-Rx subclone of E. coli has been disseminated among ST131 strains in combination with beta lactamase subtype CTX-15that may play a role in the prevalence of antibiotic resistance among E. coli strains that cause extra-intestinal infections. This study has been done to investigate the virulence factors of ST131 with their subclones: H30-Rx and non H30-Rx strains. A hundred E. coli isolates which were isolated from different clinical samples were collected from K.R hospital, Mysore. Polymerase chain reaction (PCR) method was used to investigate 19 of the virulence factors among E. coli stains. Out of 100 E. coli strains, 87 of the isolates belonged to ST131, 24 isolates belonged to H30 subclone, and 22/24 (91.7%) belonged to H30-Rx subcolne. ST131 strains harbored high virulence factors more than non ST131 strains and the differences between them were significant for papC, papEF and fyuA genes. All H30-Rx strains were positive for iutA and fimH, however, 95.5% and 81.8% of H30-Rx strains were positive for fyuA and kpsMII respectively and the differences between H30-Rx and non H30-Rx strains were significant for those genes. There are no more reports about H30 and H30-Rx subclones of ST131 E. coli in India. Further studies should be done to investigate the prevalence of ST131 in different places in India and to highlight the other virulence factors to control the dissemination of ST131 clone.

Item Type:	Article
Subjects:	B Life Science > Microbiology
Divisions:	Department of > Microbiology
Depositing User:	Manjula P Library Assistant
Date Deposited:	03 Jun 2019 07:56
Last Modified:	01 Jul 2019 07:48
URI:	http://eprints.uni-mysore.ac.in/id/eprint/2095

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