Evaluation of efficiency of hemi-nested PCR assay for the detection of 'Candidatus Liberibacter' infecting citrus

Sahana Bhaskara, A. and Jawad Ahmed, B. N. and Adkar-Purushothama, C. R. and Nagendra Prasad, M. N. and Sreenivasa, M. Y. and Maheshwar, P. K. (2013) Evaluation of efficiency of hemi-nested PCR assay for the detection of 'Candidatus Liberibacter' infecting citrus. Journal of Plant Diseases and Protection, 120 (5/6). pp. 189-193. ISSN 1861-3837

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Abstract

The present study reports the development and evaluation of a hemi-nested polymerase chain reaction (hnPCR) assay for the efficient detection of 'Candidatus Liberibacter' infect- ing citrus plants. A set of new primers was designed by alignment of nucleotide sequences of the ß-operon (rplKAJL- rpoBC) ribosomal protein genes from all the known ‘Candi- datus Liberibacter asiaticus' isolates reported in Genbank. Hemi-nested PCR reaction components and thermal cycling parameters were optimized and reaction conditions were standardized. Sequencing of the PCR products from hemi nested-PCR reactions confirmed the specificity of new primer pairs for ‘Candidatus Liberibacter asiaticus'. The reliability and sensitivity of hnPCR was evaluated by comparing it to real-time PCR.

Item Type: Article
Subjects: Agriculture Biological Sciences > Microbiology
University of Mysore > PG Campuses > Manasagangotri, Mysore > Agriculture Biological Sciences > Microbiology
Divisions: Constituent Colleges > Yuvaraja's College Mysore > Microbiology
PG Campuses > Manasagangotri, Mysore > Microbiology
Depositing User: Praveen Kumari B. L.
Date Deposited: 02 Mar 2018 09:54
Last Modified: 02 Mar 2018 09:54
URI: http://eprints.uni-mysore.ac.in/id/eprint/20947

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