Endo-symbiont mediated synthesis of gold nanobactericides and their activity against human pathogenic bacteria

Syed, Baker and Nagendra Prasad, M. N. and Mohan Kumar, K. and Dhananjaya, B. L. and Satish, S. (2017) Endo-symbiont mediated synthesis of gold nanobactericides and their activity against human pathogenic bacteria. Environmental Toxicology and Pharmacology, 52. pp. 143-149. ISSN 1382-6689

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Official URL: https://doi.org/10.1016/j.etap.2017.03.016

Abstract

Synthesis of gold nanobactericides (AuNBs) were achieved by treating 1 mM chloroaurate with cell free supernatant of Aneurinibacillus migulanus. Formation of AuNBs was initially was monitored with change in colour to ruby red. Further confirmation was assessed with UV–visible spectra with maximum absorption occurring at 510 nm. Transmission electron microscopy (TEM) analysis revealed the polydispersity of AuNBs with size distribution ranging from 10 to 60 nm with an average size of 30 nm. Crystalline nature was studied using X-ray diffraction which exhibited characteristic peaks indexed to Bragg's reflection at 2θ angle which confers (111), (200), (220), and (311) planes suggesting AuNBs were face-centred cubic. Fourier transform infrared spectroscopy (FTIR) analysis revealed absorption peaks occurring at 3341 cm−1, 1635 cm−1 and 670 cm−1 which corresponds to functional groups attributing to synthesis. The antibacterial efficacy of AuNBs was tested against selective human pathogenic bacteria and activity was measured as zone of inhibition by using disc and well diffusion. Bactericidal activity was interpreted with standard antibiotics gentamicin and kanamycin. Micro broth dilution assay expressed the minimal concentration of AuNBs to inhibit the growth of test pathogens. Highest activity was observed against Pseudomonas aeruginosa (MTCC 7903) with 21.00 ± 0.57 mm compared to other pathogens. The possible mode of action of AuNBs on DNA was carried out with in vitro assay as preliminary test against pathogenic DNA isolated from P. aeruginosa. Further studies will be interesting enough to reveal the exact interactive mechanism of AuNBs with DNA. Overall study contributes towards biogenic synthesis of AuNBs as one of the alternative in combating drug resistant pathogens.

Item Type: Article
Additional Information: Unmapped bibliographic data: DA - 2017/06/01/ [EPrints field already has value set] JO - Environmental Toxicology and Pharmacology [Field not mapped to EPrints]
Uncontrolled Keywords: Gold nanobactericides, , L., Bactericidal activity, Human pathogens
Subjects: Agriculture Biological Sciences > Microbiology
University of Mysore > PG Campuses > Manasagangotri, Mysore > Agriculture Biological Sciences > Microbiology
Divisions: PG Campuses > Manasagangotri, Mysore > Microbiology
Depositing User: Praveen Kumari B.L
Date Deposited: 16 Jan 2018 09:10
Last Modified: 16 Jan 2018 09:10
URI: http://eprints.uni-mysore.ac.in/id/eprint/20650

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