Evaluation of efficiency of hemi-nested PCR assay for the detection of `candidatus liberibacter' infecting citrus

Bhaskara, A. S. and Ahmed, B. N. J. and Adkar-Purushothama, C. and Prasad, N. N. and Sreenivasa, M. Y. and Maheshwar, P. (2013) Evaluation of efficiency of hemi-nested PCR assay for the detection of `candidatus liberibacter' infecting citrus. Journal of Plant Diseases and Protection, 120 (5-6). pp. 189-193. ISSN 1861-3837

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Abstract

The present study reports the development and evaluation of a hemi-nested polymerase chain reaction (hnPCR) assay for the efficient detection of `Candidatus Liberibacter' infecting citrus plants. A set of new primers was designed by alignment of nucleotide sequences of the beta-operon (rp1KAJL-rpoBC) ribosomal protein genes from all the known `Candidatus Liberibacter asiaticus' isolates reported in Genbank. Hemi-nested PCR reaction components and thermal cycling parameters were optimized and reaction conditions were standardized. Sequencing of the PCR products from hemi nested-PCR reactions confirmed the specificity of new primer pairs for `Candidatus Liberibacter asiaticus'. The reliability and sensitivity of hnPCR was evaluated by comparing it to real-time PCR.

Item Type: Article
Uncontrolled Keywords: hnPCR; Huanglongbing; Liberibacter; ribosomal protein gene
Subjects: B Life Science > Microbiology
Divisions: Department of > Microbiology
Depositing User: Users 23 not found.
Date Deposited: 04 Jun 2019 09:48
Last Modified: 12 Jul 2022 09:54
URI: http://eprints.uni-mysore.ac.in/id/eprint/2049

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