Spectrophotometric assay of diethylcarbamazine citrate in pharmaceuticals and spiked human urine by ion-pair reaction with Tropaeolin 000

Basavaiah, K. and Qarah Nagib, A. S. (2016) Spectrophotometric assay of diethylcarbamazine citrate in pharmaceuticals and spiked human urine by ion-pair reaction with Tropaeolin 000. Jordan Journal of Chemistry, 11 (3). pp. 159-172.

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Abstract

A simpleand rapid extractive spectrophotometric method is presented for the determination of diethylcarbamazine citrate (DEC) in bulk drug, dosage forms and spiked human urine. The method is based on the formation of orangecoloredion-pair complex betweenthebasic DEC and the acidic dye Tropaeolin 000 (TRN) in Clarks-Lubs buffer of pH 1.42. The formed complex was extracted bychloroform and the absorbance was measured at 490 nm. The effectof variousexperimental parameters on complex formation wasinvestigated and optimized. Beer’s law is obeyed over the concentration range 2.0-70 μg mL-1(R=0.9995) with a molar absorptivity of 5.207×103L mol-1cm-1. The limitsof detection (LOD) and quantification (LOQ) were calculated to be 0.15 and 0.46 μg mL-1, respectively. The composition of the ion-pair was found to be 1:1 (DEC:TRN) by Job’s method of continuous variations. The relative standard deviation (%RSD)for intra-day and inter-day precision wasless than 1.5% and the relative error (%RE) wasbetter than 2.0%. The method was applied to the determination of DEC in tablets andsyrupand the results agreedwell with the label claim. The accuracy of the method was confirmed by recovery study viastandard-addition procedure. In virtue of its high sensitivity, the method was applied to spiked human urine andan excellent percent recovery of 96.38±1.64was obtained.

Item Type:	Article
Subjects:	C Chemical Science > Chemistry
Divisions:	Department of > Chemistry
Depositing User:	Manjula P Library Assistant
Date Deposited:	01 Jun 2019 06:25
Last Modified:	01 Jun 2019 06:25
URI:	http://eprints.uni-mysore.ac.in/id/eprint/2014

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