Biogenic nanoparticles bearing antibacterial activity and their synergistic effect with broad spectrum antibiotics: Emerging strategy to combat drug resistant pathogens

Baker, Syed and Pasha, Azmath and Satish, S. (2017) Biogenic nanoparticles bearing antibacterial activity and their synergistic effect with broad spectrum antibiotics: Emerging strategy to combat drug resistant pathogens. Saudi Pharmaceutical Journal, 25 (1). pp. 44-51. ISSN 1319-0164

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Official URL: https://doi.org/10.1016/j.jsps.2015.06.011

Abstract

The present study emphasizes on synthesis of bimetallic silver–gold nanoparticles from cell free supernatant of Pseudomonas veronii strain AS41G inhabiting Annona squamosa L. The synthesized nanoparticles were characterized using hyphenated techniques with UV–Visible spectra ascertained absorbance peak between 400 and 800 nm. Possible interaction of biomolecules in mediating and stabilization of nanoparticles was depicted with Fourier transform infrared spectroscopy (FTIR). X-ray diffraction (XRD) displayed Bragg’s peak conferring the 1 0 0, 1 1 1, 2 0 0, and 2 2 0 facets of the face centered cubic symmetry of nanoparticles suggesting that these nanoparticles were crystalline in nature. Size and shape of the nanoparticles were determined using Transmission electron microscopy (TEM) microgram with size ranging from 5 to 50 nm forming myriad shapes. Antibacterial activity of nanoparticles against significant human pathogens was conferred with well diffusion assay and its synergistic effect with standard antibiotics revealed 87.5% fold increased activity with antibiotic “bacitracin” against bacitracin resistant strains Bacillus subtilis, Escherichia coli and Klebsiella pneumoniae followed by kanamycin with 18.5%, gentamicin with 11.15%, streptomycin with 10%, erythromycin with 9.7% and chloramphenicol with 9.4%. Thus the study concludes with biogenic and ecofriendly route for synthesizing nanoparticles with antibacterial activity against drug resistant pathogens and attributes growing interest on endophytes as an emerging source for synthesis of nanoparticles.

Item Type: Article
Additional Information: Unmapped bibliographic data: DA - 2017/01/01/ [EPrints field already has value set] JO - Saudi Pharmaceutical Journal [Field not mapped to EPrints]
Uncontrolled Keywords: Biogenic, Bimetallic silver–gold nanoparticles, AS41G, Synergistic activity, Broad spectrum antibiotics
Subjects: Agriculture Biological Sciences > Microbiology
University of Mysore > PG Campuses > Manasagangotri, Mysore > Agriculture Biological Sciences > Microbiology
Divisions: PG Campuses > Manasagangotri, Mysore > Microbiology
Depositing User: Praveen Kumari B.L
Date Deposited: 28 Oct 2017 09:16
Last Modified: 28 Oct 2017 09:16
URI: http://eprints.uni-mysore.ac.in/id/eprint/20111

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