In vivo toxicity studies on gall extracts of Terminalia chebula (Gaertn.) Retz. (combretaceae)

Ravi Shankara, B. E. and Ramachandra, Y. L. and Sundara Rajan, S. and Sujan Ganapathy, P. S. and Austin, Richard Surendranath and Dhananjaya, B. L. (2016) In vivo toxicity studies on gall extracts of Terminalia chebula (Gaertn.) Retz. (combretaceae). Pharmacognosy Research, 8 (3). pp. 199-201.

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The galls of Terminala chebula (Gaertn.) Retz. (Combretaceae) are used for the treatment of various diseases in folk medicine and has been found to posses anti-inflammatory, anti-bacterial, anti-helmintic, anti-tyrosinase, and anti-aging activities. Considering the ethano-botanical and diverse pharmacological applications of galls of T. chebula, in this study, we investigate the possible toxic effects of different gall extracts of T. chebula by Brine shrimp (Artemia salina) toxicity assay. The cytotoxicity test of leaf gall extracts (petroleum ether, chloroform, ethanol, and aqueous) of T. chebula was evaluated by Brine shrimp (A. salina) toxicity assay, which is based on the ability to kill laboratory cultured Artemia nauplii (animals eggs) and also total content of polyphenols, flavonoids with other qualitative phytochemical analysis of the extract were determined. It was observed that the petroleum ether extract was virtually nontoxic on the shrimps, and exhibited very low toxicity with LC50value of 4356.76 μg/ml. Furthermore, the chloroform extract exhibited very low toxicity, giving LC50value of 1462.2 μg/ml. On the other hand, the ethanol extract was very toxic to brine shrimps with LC50value of 68.64 μg/ml. The ethanol extract had the highest total phenolic and flavonoid content of 136 ± 1.5 mg of gallic acid equivalent/g d.w and 113 ± 1.6 mg of quercetin equivalent/g d.w, respectively. The higher toxicity effect was positively correlated to the high content of total polyphenols/flavonoids in the extract. This significant lethality of different extracts to brine shrimp is an indicative of the presence of potent cytotoxic components which warrants further investigation.

Item Type: Article
Additional Information: Unmapped bibliographic data: JO - Phcog Res [Field not mapped to EPrints]
Subjects: Agriculture Biological Sciences > Biochemistry
University of Mysore > PG Campuses > Manasagangotri, Mysore > Agriculture Biological Sciences > Biochemistry
Divisions: PG Campuses > Manasagangotri, Mysore > Biochemistry
Depositing User: Praveen Kumari B.L
Date Deposited: 08 Sep 2017 05:36
Last Modified: 08 Sep 2017 05:36

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