Nano-aptamer based quantitative detection of chloramphenicol

Sharma, Richa and Ragavan, K. V. and Raghavarao, K. S. M. S. and Thakur, M. S. (2016) Nano-aptamer based quantitative detection of chloramphenicol. In: Biotechnology and Biochemical Engineering. Springer , Singapore, pp. 187-195. ISBN 978-981-10-1920-3

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Chloramphenicol (CAP) is a broad-spectrum antibiotic, chronic use of which leads to aplastic anaemia and bone marrow suppression. Most countries, including India, have banned CAP from animal food production. However, it has still been detected in milk, honey and aquaculture products. The existing screening methods for CAP are time-consuming, laborious and expensive. Hence, there exists a need to develop an economical, fast and convenient method. In the current technique, single-stranded oligonucleotide molecule that binds specifically to CAP (aptamer) was used to protect synthesized gold nanocolloids from salt-induced aggregation, leading to colour change. The prepared nanocolloids were of size 7 nm and concentration 1.83 × 10−8 M. The buffer, salt, aptamer concentrations and incubation time were optimized. With optimized parameters, the proposed system could detect CAP up to 0.55 pg/mL in buffer, with a linear range of detection from 0.55 pg/mL to 55 μg/mL. The detection time was 30 min and cost was minimal (approximately Rs. 20 for 10 assays). Such a biosensing format, if extrapolated to real samples, can be used for rapid and effective quantitative screening for CAP in food matrices.

Item Type: Book Section
Additional Information: Unmapped bibliographic data: ED - B. D., Prasanna [Field not mapped to EPrints] ED - Gummadi, Sathyanarayana N [Field not mapped to EPrints] ED - Vadlani, Praveen V. [Field not mapped to EPrints] BT - Biotechnology and Biochemical Engineering: Select Proceedings of ICACE 2015 [Field not mapped to EPrints]
Subjects: Physical Sciences > Material Science
Divisions: PG Campuses > Manasagangotri, Mysore > Material Science
Depositing User: Praveen Kumari B.L
Date Deposited: 23 Aug 2017 06:55
Last Modified: 23 Aug 2017 06:55

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