Escherichia coli Braun Lipoprotein (BLP) exhibits endotoxemia – like pathology in Swiss albino mice

Lakshmikanth, C. L. and Jacob, Shancy Petsel and Kudva, Avinash Kundadka and Latchoumycandane, Calivarathan and Yashaswini, P. S. and Sumanth, M. S. and Goncalves-de-Albuquerque, Cassiano F. and Silva, Adriana R. and Sridevi Annapurna Singh, and Castro-Faria-Neto, Hugo C. and Prabhu, S. K. and McIntyre, Thomas M. and Marathe, Gopal Kedihithlu (2016) Escherichia coli Braun Lipoprotein (BLP) exhibits endotoxemia – like pathology in Swiss albino mice. Scientific Reports, 6. ISSN 2045-2322

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The endotoxin lipopolysaccharide (LPS) promotes sepsis, but bacterial peptides also promote inflammation leading to sepsis. We found, intraperitoneal administration of live or heat inactivated E. coli JE5505 lacking the abundant outer membrane protein, Braun lipoprotein (BLP), was less toxic than E. coli DH5α possessing BLP in Swiss albino mice. Injection of BLP free of LPS purified from E. coli DH5α induced massive infiltration of leukocytes in lungs and liver. BLP activated human polymorphonuclear cells (PMNs) ex vivo to adhere to denatured collagen in serum and polymyxin B independent fashion, a property distinct from LPS. Both LPS and BLP stimulated the synthesis of platelet activating factor (PAF), a potent lipid mediator, in human PMNs. In mouse macrophage cell line, RAW264.7, while both BLP and LPS similarly upregulated TNF-α and IL-1β mRNA; BLP was more potent in inducing cyclooxygenase-2 (COX-2) mRNA and protein expression. Peritoneal macrophages from TLR2(−/−) mice significantly reduced the production of TNF-α in response to BLP in contrast to macrophages from wild type mice. We conclude, BLP acting through TLR2, is a potent inducer of inflammation with a response profile both common and distinct from LPS. Hence, BLP mediated pathway may also be considered as an effective target against sepsis.

Item Type: Article
Additional Information: Unmapped bibliographic data: PY - 2016/10/04 [EPrints field already has value set] PY - 2016/04/01/received [EPrints field already has value set] PY - 2016/09/15/accepted [EPrints field already has value set] U1 - 27698491[pmid] [Field not mapped to EPrints] DB - PMC [Field not mapped to EPrints] AN - PMC5048175 [Field not mapped to EPrints] J1 - Sci Rep [Field not mapped to EPrints]
Subjects: Agriculture Biological Sciences > Biochemistry
University of Mysore > PG Campuses > Manasagangotri, Mysore > Agriculture Biological Sciences > Biochemistry
Divisions: PG Campuses > Manasagangotri, Mysore > Biochemistry
Depositing User: Praveen Kumari B.L
Date Deposited: 18 Aug 2017 09:18
Last Modified: 18 Aug 2017 09:18

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