PGP potential, abiotic stress tolerance and antifungal activity of Azotobacter strains isolated from paddy soils

Chennappa, G. and Naik, M. K. and Adkar-Purushothama, C. R. and Amaresh, Y. S. and Sreenivasa, M. Y. (2016) PGP potential, abiotic stress tolerance and antifungal activity of Azotobacter strains isolated from paddy soils. Indian Journal of Experimental Biology, 54. pp. 322-331. ISSN 0019-5189

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Abstract

Azotobacter strains were isolated by serial dilution method and colonies were viscous, smooth, glistening, and brown to black colour on Jenson’s N-free agar. Morphological and biochemical tests showed characteristic features of Azotobacter. Further, molecular analyses revealed the presence of different Azotobacter species viz., A. armeniacus, A. chroococcum, A. salinestris, A. tropicalis and A. vinelandii. The isolates were tested for their ability of nitrogen fixation, indole acetic acid (IAA), gibberllic acid production and phosphate solubilization. Four isolates (GVT-1, GVT-2 KOP-11 and SND-4) were efficient in fixation of highest amount of N2 (29.21 μg NmL-1day-1), produced IAA (25.50 µg mL-1), gibberllic acid (17.25 μg 25 mL-1) and formed larger P solubilizing zone (13.4 mm). Some of the Azotobacter strains were produced siderophores, hydrogen cyanide and were positive for ammonia production with respect to antifungal activity of Azotobacter was tested with dual culture method and A. tropicalis inhibited the growth of Fusarium, Aspergillus and Alternaria species. Azotobacter isolates were tested against salt (0-10%), temperature (4-55ºC), pH (5.0-10) and insecticide chloropyrifos (0-3%) tolerance study. Among them, A. chroococcum was found tolerant to a maximum of 6% NaCl with a temperature of 35-45ºC and to a pH up to 8. All the 4 strains showed effective growth against 3% chloropyrifos concentration. The studies revealed that the Azotobacter strains not only produced plant growth promoting substances but are also tolerant to abiotic stresses such as temperature, pH and insecticides.

Item Type: Article
Subjects: Agriculture Biological Sciences > Microbiology
University of Mysore > PG Campuses > Manasagangotri, Mysore > Agriculture Biological Sciences > Microbiology
Divisions: PG Campuses > Manasagangotri, Mysore > Microbiology
Depositing User: Praveen Kumari B.L
Date Deposited: 27 Jun 2017 05:49
Last Modified: 27 Jun 2017 05:49
URI: http://eprints.uni-mysore.ac.in/id/eprint/19579

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