Albizia lebbeck seed methanolic extract as a complementary therapy to manage local toxicity ofEchis carinatus venom in a murine model

Amog, P. U. and Manjuprasanna, V. N. and Yariswamy, M. and Nanjaraj Urs, A. N. and Joshi, Vikram and Suvilesh, K. N. and Nataraju, A. and Vishwanath, B. S. and Gowda, T. V. (2016) Albizia lebbeck seed methanolic extract as a complementary therapy to manage local toxicity ofEchis carinatus venom in a murine model. Pharmaceutical Biology, 54 (11). pp. 2568-2574. ISSN 1744-5116

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Official URL: http://dx.doi.org/10.3109/13880209.2016.1171882

Abstract

Viperid venom-induced chronic local-toxicity continues even after anti-snake venom treatment. Therefore, traditional antidote Albizia lebbeck L. (Fabaceae) seed extract was tested against Echis carinatus S. (Viperidae) venom (ECV)-induced local toxicity to evaluate its complementary remedy. Materials and methods: Soxhlet extraction of A. lebbeck seeds was performed with the increasing polarity of solvents (n-hexane to water); the extract was screened for phytochemicals (alkaloids, anthraquinones, flavonoids, glycosides, phenolics, saponins, steroids and tannins). In preliminary in vitro analysis, A. lebbeck methanolic extract (ALME) demonstrated significant inhibition of ECV proteases, the major enzyme–toxin responsible for local- toxicity. Therefore, in vitro neutralizing potential of ALME was further evaluated against hyaluronidases and phospholipase A2 (1:1–1:100 w/w). In addition, alleviation of ECV induced characteristic local- toxicity [haemorrhage (i.d.) and myotoxicity (i.m.)] was determined in mice.

Item Type: Article
Subjects: Agriculture Biological Sciences > Biochemistry
University of Mysore > PG Campuses > Manasagangotri, Mysore > Agriculture Biological Sciences > Biochemistry
Divisions: PG Campuses > Manasagangotri, Mysore > Biochemistry
Depositing User: Ms. Manjula p
Date Deposited: 03 Jun 2017 06:24
Last Modified: 03 Jun 2017 06:24
URI: http://eprints.uni-mysore.ac.in/id/eprint/19510

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