First report on molecular and biochemical variations among the populations of Fusarium oxysporum infecting tobacco in Karnataka, India

Sumana, K. and Punith, B. D. and Devaki, N. S. (2014) First report on molecular and biochemical variations among the populations of Fusarium oxysporum infecting tobacco in Karnataka, India. Journal of Agricultural Technology, 10 (4). pp. 931-950. ISSN 1686-9141

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Genetic diversity in Fusarium oxysporum f. sp. nicotianae (FON) was investigated and isolated from Hunsur tract of Karnataka, India was studied. The variability of FON isolates were investigated using Random Amplified Polymorphic DNA (RAPD), electrophoretic studies of total protein and isozymes (catalase, cellulase, peroxidase, esterase and protease). Amplification of genomic DNA of 5 isolates with OPA (1- 10) decamer primer series generated 380 polymorphic markers. Based on UPGMA analysis of RAPD, the isolates were delineated to two groups. Group 1 represented isolates 1 FON, 3 FON, 10 FON and 13 FON and group 2 represented the isolate 9 FON which were differing in cultural characteristics (data not shown) indicating the genetic diversity. The isolates were also investigated using electrophoretic studies of total protein and isozymes. The SDS PAGE revealed that, each of the FON isolates was unique in band patterns. The isozyme profiles revealed 78 scorable polymorphic bands and the data was subjected to UPGMA analysis. The isolates were delineated into 2 main groups. Group A contained isolates 3 FON, 1 FON, 13 FON and isolate 9 FON and 10 FON in group B. The spectrophotometric assay revealed that the 13 FON exhibited highest activity of 26.56 units/ml in catalase, 0.09 min/ml in peroxidase and 0.0242 units/ml in protease. Continuous spectrophotometric assay for esterase enzyme revealed highest activity in 13 FON isolate. Results showed that the estimated intraspecific variations were more pronounced with isozyme analysis than with protein markers. Isolate 13 FON was found to be entirely varying in the isozyme band patterns all through. In conclusion, cluster analysis of the DNA RAPD, protein banding patterns by SDS-PAGE, and UPGMA analysis of isozyme banding patterns were found to be efficient and effective tools for finding the genetic variability among the isolates isolated in the same geographical area and environmental conditions.

Item Type: Article
Subjects: Agriculture Biological Sciences > Biology
University of Mysore > PG Campuses > Manasagangotri, Mysore > Agriculture Biological Sciences > Biology
Divisions: Constituent Colleges > Yuvaraja's College Mysore > Biology
Depositing User: Praveen Kumari B.L
Date Deposited: 17 Dec 2015 05:47
Last Modified: 28 Jan 2016 06:08

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