Antioxidative Role of Buffalo (Bubalus bubalis) Colostrum Whey Derived Peptides During Oxidative Damage

Ashok, N. R. and Vivek, K. H. and Aparna, H. S. (2019) Antioxidative Role of Buffalo (Bubalus bubalis) Colostrum Whey Derived Peptides During Oxidative Damage. nternational Journal of Peptide Research and Therapeutics, 25 (4). pp. 1501-1508. ISSN 1573-3149

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Abstract

Nutritional and functional properties of dietary proteins are attributed to physiologically active peptides encrypted within them. In the present study, we focused on whey proteins from buffalo colostrum and analyzed the effect of peptides derived from peptic-digestion on DNP-induced oxidative stress on blood components. Peptides with multifunctional properties were predicted through the BIOPEP database. Free and transglutaminase-interlinked peptides were tested for their efficacy as antioxidants. Free peptides were found to be proficient quenchers compared to interlinked peptides. The hydrolysate (IC50-481 µg) and its permeate (< 3 kDa; IC50–505 µg) were effective in bringing elevated levels of ROS, H2O2 & catalase back to normal levels. Additionally, it also replenished the depleted levels of blood GSH and moderately restored lysosomal enzyme activities. The lead peptide-YVHPFHL sequence with the key terminal HL domain (IC50 339 ± 2 µM) obtained from permeate further substantiated the antioxidative potency of colostrum peptides. Hence, we propose that whey-based peptides can be leveraged for the production of functional foods to treat diverse physiological anomalies.

Item Type:	Article
Uncontrolled Keywords:	BIOPEP; Buffalo colostrum; DNP; Oxidative stress; Peptides; Whey proteins
Subjects:	B Life Science > Biotechnology
Divisions:	Department of > Biotechnology
Depositing User:	C Swapna Library Assistant
Date Deposited:	20 Nov 2025 09:23
Last Modified:	20 Nov 2025 09:23
URI:	http://eprints.uni-mysore.ac.in/id/eprint/17946

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