Phytoconstituents from ( Piper nigrum L.) Black pepper essential oil (BPEO) acts as anti-virulent to fight quorum sensing, an analysis through in-vitro and in-silico studies

Chatterjee, B. and Renganathan, R. R. A. and Vittal, R. R. (2025) Phytoconstituents from ( Piper nigrum L.) Black pepper essential oil (BPEO) acts as anti-virulent to fight quorum sensing, an analysis through in-vitro and in-silico studies. Industrial Crops & Products, 225. ISSN 0926-6690

Text (Full Text) Phytoconstituents from (Piper nigrum L.) Black pepper essential oil.pdf - Published Version Restricted to Registered users only Download (10MB) | Request a copy

Abstract

Sessile biofilms make microorganisms drug-resistant, which is an escalating challenge to human health. Essential oils (EOs) derived from plants and spices like Piper nigrum L. (Black Pepper) have potent anti-virulent properties. This study investigates the leading quorum-quenching (QQ) phytoconstituents in Black Pepper EO (BPEO) and their specific anti-virulent actions against Chromobacterium violaceum 12472 and Pseudomonas aeruginosa PAO1. BPEO exhibited predominant bactericidal effect at Minimal Inhibitory Concentrations (MIC) of 3 % and 6 % (+1.56 v/v) against each species respectively and inhibited quorum-sensing (QS)-regulatory phenotypic expressions at sublethal concentrations. The visible deformation of a pre-formed P. aeruginosa PAO1 biofilm-matrix after BPEO administration was observed via epifluorescence microscopy. BPEO also inhibited the production of exopolysaccharide (EPS), violacein, and migration of a motile flagellum. Thin Layer Chromatography (TLC); followed by bioautography confirmed the location of bio-active phytoconstituents at Rf 0.8, and further analyzed through Gas Chromatography/Mass Spectroscopy (GC/MS) to identify all phytoconstituents in BPEO. Virtual screening-based in-silico molecular docking revealed two leading ligands and the active pockets for these ligands in QS regulation proteins LasR and CviR that had binding energies of -8.3 and -8.6 Kcal/mol respectively. 100 nanoseconds of molecular dynamics (MD) simulation also revealed that alpha-cubebene had stronger binding stability with LasR and CviR proteins than beta-caryophyllene. Overall, this study has assessed BPEO as a potential reservoir of effective QQ molecules that inhibit biofilm-formation through QS regulation and characterized the specific biochemical interactions that define BPEO as a sustainable anti-virulent against certain drug-resistant pathogenic bacteria.

Item Type:	Article
Uncontrolled Keywords:	Quorum sensing (QS) regulation, Black pepper essential oil (BPEO) LasR, CviR, Chromobacterium violaceum 12742 β-caryophyllene. α-cubebene
Subjects:	B Life Science > Microbiology
Divisions:	Department of > Microbiology
Depositing User:	Ms Varalakshmi
Date Deposited:	29 Oct 2025 06:16
Last Modified:	29 Oct 2025 06:16
URI:	http://eprints.uni-mysore.ac.in/id/eprint/17880

Actions (login required)

View Item