Antioxidant, antibacterial, and cytoprotective activity of agathi leaf protein

Zarena, A. S. and Shubha Gopal, and Vineeth, R. (2014) Antioxidant, antibacterial, and cytoprotective activity of agathi leaf protein. Journal of Analytical Methods in Chemistry. ISSN 2090-8873

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Official URL: http://dx.doi.org/10.1155/2014/989543

Abstract

In the present study a protein termed agathi leaf protein (ALP) from Sesbania grandiflora Linn. (agathi) leaves was isolated after successive precipitation with 65% ammonium sulphate followed by purification on Sephadex G 75. The column chromatography of the crude protein resulted in four peaks of which Peak I (P I) showed maximum inhibition activity against hydroxyl radical. SDS-PAGE analysis of P I indicated that the molecular weight of the protein is ≈29 kDa. The purity of the protein was 98.4% as determined by RP-HPLC and showed a single peak with a retention time of 19.9 min. ALP was able to reduce oxidative damage by scavenging lipid peroxidation against erythrocyte ghost (85.50 ± 6.25%), linolenic acid (87.67 ± 3.14%) at 4.33 μM, ABTS anion (88 ± 3.22%), and DNA damage (83 ± 4.20%) at 3.44 μM in a dose-dependent manner. The purified protein offered significant protection to lymphocyte (72% at 30 min) induced damage by t-BOOH. In addition, ALP showed strong antibacterial activity against Pseudomonas aeruginosa (20 ± 3.64 mm) and Staphylococcus aureus (19 ± 1.53 mm) at 200 μg/mL. The safety assessment showed that ALP does not induce cytotoxicity towards human lymphocyte at the tested concentration of 0.8 mg/mL.

Item Type: Article
Additional Information: Unmapped bibliographic data: PY - 2014/// [EPrints field already has value set] M3 - 10.1155/2014/989543 [Field not mapped to EPrints] JA - J. Anal. Methods Chem. [Field not mapped to EPrints]
Uncontrolled Keywords: Sesbania leaf protein antioxidant antibacterial cytoprotectant oxidative DNA damage
Subjects: Agriculture Biological Sciences > Microbiology
University of Mysore > PG Campuses > Manasagangotri, Mysore > Agriculture Biological Sciences > Microbiology
Divisions: PG Campuses > Manasagangotri, Mysore > Microbiology
Depositing User: Praveen Kumari B.L
Date Deposited: 16 May 2015 10:36
Last Modified: 16 May 2015 10:36
URI: http://eprints.uni-mysore.ac.in/id/eprint/17876

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