Piperazine selenium nanoparticle (Pipe@SeNP's): A futuristic anticancer contender against MDA-MB-231 cancer cell line

Nagendra Prasad, H. S. and Ananda, A. P. and Sumathi, S. and Swathi, K. and Rakesh, K. J. and Jayanth, H. S. and Mallu, P. (2022) Piperazine selenium nanoparticle (Pipe@SeNP's): A futuristic anticancer contender against MDA-MB-231 cancer cell line. Journal of Molecular Structure, 1268. ISSN 1872-8014

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Official URL: https://doi.org/10.1016/j.molstruc.2022.133683


Drug resistance is a major drawback of breast cancer management mainly in treatment failure. The main objective of the current study is to investigate whether Piperazine selenium nanoparticles (Pipe@SeNP's) can induce apoptosis in breast cancer cell lines. Piperazine molecule (pipe) was synthesized and char-acterized using different spectral studies such as 1H-NMR, 13C-NMR, LCMS, and FTIR. Pipe@SeNP's was synthesized and amply characterized with UV-Vis spectra, FT-IR, SEM, EDX, XRD, and DLS highest size distribution was found to be 26.39 nm compared to other sizes with 3.8 mV zeta potential value. Before the synthesis of the Pipe@SeNP's, the core chemical was assessed for their Pharmacophore model, PASS, ADME, and BBB. All the computational assays strongly indicated the potency of the molecule. Pipe@SeNP's was treated with both normal (PBMC) and cancerous (MDA-MB-23) cell lines and investigated their dose optimization, and cytotoxicity by MTT and SRB assays. The colony morphological changes and cell prolif-eration inhibitory effect of Pipe@SeNP's were monitored up to 8 hrs against cancer and normal cell lines compared to etoposide drug with colony formation and scratch wound assay. The efficacy of normal and cancerous cell death patterns was examined in a flow cytometer. The data showed that 61.92% of cells were dead in the late apoptotic phase and 22.60% of cells in the early apoptotic stage compared to stan-dard drug etoposide 13.41% of cells were in the early apoptotic stage, 47.48% of cells in the late apoptotic phase and 20.65% of cells undergo necrosis. This revealed data strongly indicated that, Pipe@SeNP's could be of great potential in futuristic drug development against breast cancer cells. (C) 2022 Elsevier B.V. All rights reserved.

Item Type: Article
Uncontrolled Keywords: Piperazine selenium nanoparticles; Colony formation assay; Scratch wound assay; MDA-MB-23 cell line; Flow cytometry
Subjects: B Life Science > Microbiology
Divisions: Yuvaraj college > Mircobiology
Depositing User: C Swapna Library Assistant
Date Deposited: 03 Oct 2023 07:13
Last Modified: 03 Oct 2023 07:13
URI: http://eprints.uni-mysore.ac.in/id/eprint/17756

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