Nagendra Prasad, H. S. and Ananda, A. P. and Sumathi, S. and Swathi, K. and Rakesh, K. J. and Jayanth, H. S. and Mallu, P. (2022) Piperazine selenium nanoparticle (Pipe@SeNP's): A futuristic anticancer contender against MDA-MB-231 cancer cell line. Journal of Molecular Structure, 1268. ISSN 1872-8014
Full text not available from this repository. (Request a copy)Abstract
Drug resistance is a major drawback of breast cancer management mainly in treatment failure. The main objective of the current study is to investigate whether Piperazine selenium nanoparticles (Pipe@SeNP's) can induce apoptosis in breast cancer cell lines. Piperazine molecule (pipe) was synthesized and char-acterized using different spectral studies such as 1H-NMR, 13C-NMR, LCMS, and FTIR. Pipe@SeNP's was synthesized and amply characterized with UV-Vis spectra, FT-IR, SEM, EDX, XRD, and DLS highest size distribution was found to be 26.39 nm compared to other sizes with 3.8 mV zeta potential value. Before the synthesis of the Pipe@SeNP's, the core chemical was assessed for their Pharmacophore model, PASS, ADME, and BBB. All the computational assays strongly indicated the potency of the molecule. Pipe@SeNP's was treated with both normal (PBMC) and cancerous (MDA-MB-23) cell lines and investigated their dose optimization, and cytotoxicity by MTT and SRB assays. The colony morphological changes and cell prolif-eration inhibitory effect of Pipe@SeNP's were monitored up to 8 hrs against cancer and normal cell lines compared to etoposide drug with colony formation and scratch wound assay. The efficacy of normal and cancerous cell death patterns was examined in a flow cytometer. The data showed that 61.92% of cells were dead in the late apoptotic phase and 22.60% of cells in the early apoptotic stage compared to stan-dard drug etoposide 13.41% of cells were in the early apoptotic stage, 47.48% of cells in the late apoptotic phase and 20.65% of cells undergo necrosis. This revealed data strongly indicated that, Pipe@SeNP's could be of great potential in futuristic drug development against breast cancer cells. (C) 2022 Elsevier B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Piperazine selenium nanoparticles; Colony formation assay; Scratch wound assay; MDA-MB-23 cell line; Flow cytometry |
| Subjects: | B Life Science > Microbiology |
| Divisions: | Yuvaraj college > Mircobiology |
| Depositing User: | C Swapna Library Assistant |
| Date Deposited: | 03 Oct 2023 07:13 |
| Last Modified: | 03 Oct 2023 07:13 |
| URI: | http://eprints.uni-mysore.ac.in/id/eprint/17756 |
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