Dye decolorization by Penicillium Oxalicum: application of plackett-burman experimental design to evaluate nutritional requirements

Hema, N. and Satish, S. and Suresha, S. (2014) Dye decolorization by Penicillium Oxalicum: application of plackett-burman experimental design to evaluate nutritional requirements. International Journal of Recent Scientific Research, 5 (7). pp. 1347-1352. ISSN 0976-3031

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The strain Penicillium oxalicum RF3 isolated from textile dye effluent wasevaluated for its ability to decolorize the acid dye Nitonol Green BLS. Percentage decolorization of the dye by fungi was taken as the response to screen and optimize the media constituents using statistically valid Plackett-Burman and response surface methodology (RSM) design of experiments, respectively. The significance of the factors was analyzed in the form of ANOVA, in which individual factors didnot show any significant effects. Therefore, interaction effects among factors were analyzed, which indicated that glucose - potassium dihydrogen phosphate andsodium sulphite - potassium dihyrogen phosphate showed significant effect on dyedecolorization. Response surface methodology used in optimizing the importantmedia constituents for enhancing the decolorization of Nitonol Green BLS,revealed optimum combinations of all factors which gave maximum percent dyedecolorization of 71% with incubation duration=119.99 hours, Sucrose=0.97%,Glucose=1%, Na2SO3=0.5%, MgSO4=0.96%, KH2PO4=0.52%, NH4Cl=1% and pH7, temperature 25°C, dye concentration 0.01%, inoculum size=10%. The Paretochart plotted for all seven factors indicated that incubation duration, MgSO4 andNH4Cl positively influenced the dye decolorization process. The degradation of Nitonol Green BLS into different metabolites by the isolate was confirmed usingFourier transform infrared spectroscopy. These results suggest that the isolatedfungi are suitable for the biological treatment of dye-containing was tewater.

Item Type: Article
Subjects: Agriculture Biological Sciences > Microbiology
University of Mysore > PG Campuses > Manasagangotri, Mysore > Agriculture Biological Sciences > Microbiology

Physical Sciences > Environmental Science
Divisions: Constituent Colleges > Yuvaraja's College Mysore > Environmental Science
PG Campuses > Manasagangotri, Mysore > Microbiology
Depositing User: Praveen Kumari B.L
Date Deposited: 25 Apr 2015 06:14
Last Modified: 25 Apr 2015 06:14
URI: http://eprints.uni-mysore.ac.in/id/eprint/17731

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