Protective effect of Euphorbia hirta and its components against snake venom induced lethality

Gopi, K. and Renu, Kadali and Vishwanath, B. S. and Jayaraman, Gurunathan (2015) Protective effect of Euphorbia hirta and its components against snake venom induced lethality. Journal of Ethnopharmacology, 165. pp. 180-190. ISSN 0378-8741

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Ethnopharmacological relevance Despite the use of snake anti-venom therapy, herbal medicine is still in practice to treat snakebites. Euphorbia hirta is traditionally used as antidote for snakebites and also for numerous other ailments. However, the scientific evidence for its anti-snake venom property is still lacking. Materials and methods Methanolic extract of E. hirta was evaluated for anti-venom activity under in vitro and ex vivo conditions. Histopathological changes in the vital organs of the mice were also monitored. UHPLC-SRM/MS was used to estimate the phenolic constituents whereas GC–MS analysis was performed to analyze the volatile metabolites present. The major compound was further evaluated for its contribution to the overall inhibitory potential of the extract. Results Methanolic extract of E. hirta completely inhibited the venom enzymes under in vitro and reduced the edema ratio. The extract increased the survival time (>24 h) of mice which was further evidenced by histopathological analysis of vital organs. Phytochemical analysis revealed higher content of phenolic (144 mg/g extract) compounds in the extract. UHPLC-SRM/MS demonstrated that ellagic acid, gallic acid and quinic acid are the major phenolics whereas GC–MS analysis revealed pyrogallol as the major constituent (60.07%) among the volatile components of the extract. It was also shown that pyrogallol has the ability to differentially inhibit venom protease but not phospholipase A2. Conclusion The present study confirmed that E. hirta methanolic extract was able to completely inhibit Naja naja venom induced toxicity under in vitro as well as ex vivo conditions, thus providing scientific evidence to its traditional use.

Item Type: Article
Additional Information: Unmapped bibliographic data: PY - 2015/5/13/ [EPrints field already has value set] JO - Journal of Ethnopharmacology [Field not mapped to EPrints]
Uncontrolled Keywords: Snake venom, Naja naja, Neutralization, Euphorbia hirta, Phenolic compounds, Pyrogallol
Subjects: Agriculture Biological Sciences > Biochemistry
University of Mysore > PG Campuses > Manasagangotri, Mysore > Agriculture Biological Sciences > Biochemistry
Divisions: PG Campuses > Manasagangotri, Mysore > Biochemistry
Depositing User: Ms. Swapna deepa
Date Deposited: 08 Apr 2015 07:35
Last Modified: 09 Nov 2015 09:37

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