Immunity Elicitors for Induced Resistance against the Downy Mildew Pathogen in Pearl Millet

Lavanya, S. N. and Niranjan Raj, S. and Jadimurthy, Ragi and Sudarsan, Sujesh and Srivastava, Rakesh and Tarasatyavati, C. and Rajashekara, H. and Gupta, Vijai Kumar and Nayaka, S. C. (2022) Immunity Elicitors for Induced Resistance against the Downy Mildew Pathogen in Pearl Millet. Scientific Reports, 12.

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Abstract

Pearl millet (Pennisetum glaucum (L.) R. Br.) is a globally important cereal whose production is severely constrained by downy mildew caused by Sclerospora graminicola (Sacc.). In this study, immunity eliciting properties of 3,5-dichloroanthranilic acid (DCA), Cell Wall Glucan (CWG), Lipopolysaccharide (LPS), and Glycinebetaine (GB) was deciphered through enzymatic and protein studies based on elicitor treatment activated defense mechanisms. Glycinebetaine, LPS, CWS and DCA elicited enzyme activities and gene expression of the defense enzymes, such as β-1,3-glucanase, phenylalanine ammonia lyase (PAL), peroxidase (POX), polyphenol oxidase (PPO), lipoxygenase (LOX) and defense protein hydroxyproline-rich glycoproteins (HRGPs). However, the speed and the extent of elicitation differed. High levels of enzyme activities and gene expression in elicitor-treated P. glaucum positively correlated with the increased downy mildew resistance. A very rapid and large changes in elicitor-treated seedlings, in contrast to the delayed, smaller changes in the untreated susceptible control seedlings suggests that the rate and magnitude of defense gene expression are important for effective manifestation of defense against pathogen. As compared to other elicitors and control, GB promoted increase in enzyme activities and gene expression, implicating that GB is a promising elicitor of downy mildew resistance in P. glaucum

Item Type:	Article
Subjects:	B Life Science > Biotechnology
Divisions:	Department of > Biotechnology
Depositing User:	C Swapna Library Assistant
Date Deposited:	01 Jul 2023 10:00
Last Modified:	01 Jul 2023 10:00
URI:	http://eprints.uni-mysore.ac.in/id/eprint/17561

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