Mishra, Neelam and Rana, Kavita and Deepthi Seelam, Siva and Rakesh Kumar and Pandey, Vijyendra and Bharathi P. Salimath and Agsar, Dayanand (2021) Characterization and Cytotoxicity of Pseudomonas Mediated Rhamnolipids Against Breast Cancer MDA-MB-231 Cell Line. Frontiers in Bioengineering and Biotechnology, 9. pp. 1-14.
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Abstract
A biosurfactant producing bacterium was identified as Pseudomonas aeruginosa DNM50 based on molecular characterization (NCBI accession no. MK351591). Structural characterization using MALDI-TOF revealed the presence of 12 different congeners of rhamnolipid such as Rha-C8-C8:1, Rha-C10-C8:1, Rha-C10-C10, Rha-C10-C12:1, Rha-C16:1, Rha-C16, Rha-C17:1, Rha-Rha-C10:1-C10:1, Rha-Rha-C10-C12, Rha-Rha-C10-C8, Rha-Rha-C10-C8:1, and Rha-Rha-C8-C8. The radical scavenging activity of rhamnolipid (DNM50RL) was determined by 2, 3-diphenyl-1-picrylhydrazyl (DPPH) assay which showed an IC50 value of 101.8 mu g/ ml. The cytotoxic activity was investigated against MDA-MB-231 breast cancer cell line by MTT (4,5-dimethylthiazol-2-yl-2,5-diphenyl tetrazolium bromide) assay which showed a very low IC50 of 0.05 mu g/ ml at 72 h of treatment. Further, its activity was confirmed by resazurin and trypan blue assay with IC50 values of 0.01 mu g/ml and 0.64 mu g/ ml at 72 h of treatment, respectively. Thus, the DNM50RL would play a vital role in the treatment of breast cancer targeting inhibition of p38MAPK.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | MDA-MB-231 cell lines; rhamnolipid; MTT; trypan blue; p38MAPK; TNBC; resazurin; cytotoxic |
| Subjects: | B Life Science > Biotechnology |
| Divisions: | Department of > Biotechnology |
| Depositing User: | Mr Umendra uom |
| Date Deposited: | 21 May 2022 10:01 |
| Last Modified: | 13 Jul 2022 11:01 |
| URI: | http://eprints.uni-mysore.ac.in/id/eprint/17222 |
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