Extracellular synthesis of silver nanoparticles by novel Pseudomonas veronii AS 41G inhabiting Annona squamosa L. and their bactericidal activity

Baker, Syed and Mohan Kumar, K and Santosh, P. and Rakshith, D. and Satish, S. (2015) Extracellular synthesis of silver nanoparticles by novel Pseudomonas veronii AS 41G inhabiting Annona squamosa L. and their bactericidal activity. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy. ISSN 1386-1425

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Official URL: http://dx.doi.org/10.1016/j.saa.2014.10.033

Abstract

In present investigation extracellular synthesis of silver nanoparticles were synthesized using cell free supernatant of Pseudomonas veronii AS41G isolated from Annona squamosa L. The bacterium significantly reduced silver nitrate to generate silver nanoparticles which was characterized with hyphenated techniques. Synthesis of silver nanoparticles preliminary confirmed by UV-Visible spectrophotometry with the intense peak at 410nm, Further FTIR analysis revealed the possible role of biomolecules in the supernatant responsible for mediating the nanoparticles formation. The XRD spectra exhibited the characteristic Bragg peaks of 100, 111, 200, and 220 facets of the face centred cubic symmetry of nanoparticles suggesting that these nanoparticles were crystalline in nature. TEM microgram showed polydispersity of nanoparticles with size ranging from 5 to 50 nm. Synthesized silver nanoparticles showed antibacterial activity against human and environmental pathogens including MRSA. The study enlightens the role of biosynthesized silver nanoparticles as an emerging alternative for drug resistant microorganisms. The obtained results are promising enough to pave the environmentally benign nanoparticle synthesis processes without use of any toxic chemicals and also envision the emerging role of endophytes towards synthesis of nanoparticles. With scanty reports available on Pseudomonas veronii species, a new role has been reported in this study which will be very valuable for future researchers working on it.

Item Type: Article
Additional Information: Unmapped bibliographic data: JO - Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy [Field not mapped to EPrints]
Uncontrolled Keywords: Endophyte, Pseudomonas veronii AS41G, Silver nanoparticles, Annona squamosa L
Subjects: Agriculture Biological Sciences > Microbiology
University of Mysore > PG Campuses > Manasagangotri, Mysore > Agriculture Biological Sciences > Microbiology
Divisions: PG Campuses > Manasagangotri, Mysore > Microbiology
Depositing User: ADMIN
Date Deposited: 03 Nov 2014 10:11
Last Modified: 30 Jan 2015 09:49
URI: http://eprints.uni-mysore.ac.in/id/eprint/17190

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